不同亚型肠上皮化生黏膜mtp53和c-myc蛋白表达与幽门螺杆菌细胞毒素相关基因蛋白A的关系  

The association between cytotoxin-associated gene A of Helicobacter pylori and the expressions of mtp53 and c-myc protein in different subtypes of intestinal metaplasia

作　　者：黄珏 赖跃兴 徐萍[2] 

机构地区：[1]上海市松江区九亭医院消化内科,201615 [2]上海交通大学附属第一人民医院松江分院消化内科,201600

出　　处：《中国医师进修杂志》2012年第34期4-7,共4页Chinese Journal of Postgraduates of Medicine

基　　金：上海市卫生局课题（2009251）

摘　　要：目的研究幽门螺杆菌（Hp）细胞毒素相关基因蛋白A（Hp—CagA）与肠上皮化生不同亚型中mtp53和c-mye蛋白表达的关系。方法选取胃镜检查患者165例，其中慢性萎缩性胃炎伴肠上皮化生125例，非萎缩性胃炎40例。肠上皮化生分为完全型小肠上皮化生、不完全型小肠上皮化生、完全型结肠上皮化生和不完全型结肠上皮化生四种亚型。应用14C尿素呼气试验检测Hp；通过AB．PAS和HID-AB黏液染色区分肠上皮化生亚型；应用免疫组织化学染色Elivision法检测mtp53和C．mye蛋白的表达；采用间接ELISA法检测Hp-CagA。结果不完全型结肠上皮化生45例，不完全型小肠上皮化生47例，完全型结肠上皮化生17例，完全型小肠上皮化生16例。肠上皮化生各亚型Hp-ca酣阳性率均高于非萎缩性胃炎，但差异无统计学意义（P〉0．05）。不完全型结肠上皮化生Hp．c甜阳性患者mtp53蛋白阳性表达率高于不完全型结肠上皮化生Hp-CagA阴性患者（r=5．494，P〈0．05）；不完全型结肠上皮化生Hp-cagA阳性患者c-myc蛋白阳性表达率明显高于不完全型结肠上皮化生Hp．CagA阴性患者（r=13．950，P〈0．01）。结论Hp—CagA阳性Hp是Hp的高毒力株，Hp-cagA阳性Hp具有较强的促进胃黏膜萎缩、肠上皮化生的作用，Hp—cagA阳性Hp可能通过癌基因激活实现胃黏膜肠上皮化生和癌变的过程。Objective To study the association between cytotoxin-associated gene A of Helicobacter pylori （Hp-CagA） and the expressions of mtp53 and c-myc protein in different subtypes of intestinal metaplasia. Methods One hundred and sixty-five patients with gastroscopy included 125 cases with chronic atrophic gastritis with intestinal metaplasia,40 cases with non-atrophic gastritis. Intestinal metaplasia included complete intestinal metaplasia,incompletetype intestinal metaplasia,complete colonic epithelial metaplasia and incomplete colonic epithelial metaplasia. The carbon-14-urea breath test was used to determine Helicobacter pylori （Hp） ;AB-PAS and HID-AB mucinous staining was used to distinguish intestinal metaplasia subtypes; the immunohistochemical Elivision method was used to determine the expressions of mtp53 and c-myc protein;indirect ELISA was used to determine Hp-CagA. Results Forty-five cases with incomplete colonic epithelial metaplasia,47 cases with incomplete type intestinal metaplasta, 17 cases with complete colonic epithelial metaplasia, 16 cases with complete intestinal metaplasia. The positive rate of Hp-CagA in all intestinal metaplasia subtypes was higher than that in non-atrophic gastritis, but there was no significant difference （P 〉 0.05）. The expression of mtp53 protein in incomplete colonic type of intestinal metaplasia with Hp-CagA positive was higher than that in incomplete colonic type of intestinal metaplasia with Hp-CagA negative （X^2 = 5.494 ,P 〈 0.05 ）. The expression of c-myc protein in incomplete colonic type of intestinal metaplasia with Hp-CagA positive was higher than that in incomplete colonic type of intestinal metaplasia with Hp-CagA negative （X^2 = 13.950,P 〈 0.01 ）. Conclusion Hp-CagA is a sort of highly virulent strain, and Hp-CagA may do a strong role in the promotion of gastric atrophy and intestinal metaplasia.

关 键 词：螺杆菌 幽门 原癌基因蛋白质C-MYC mtp53蛋白 细胞毒素相关基因蛋白A 不完全型结肠上皮化生 

分 类 号：R735.2[医药卫生—肿瘤]