口腔鳞癌IV型胶原和层粘连蛋白的表达与颈淋巴结转移的关系  

Expression of collagen IV and laminin in oral squamous cell carcinoma and its relationship with metastasis to neck lymph nodes

作　　者：刘刚[1] 韩德昌 严全梅[1] 

机构地区：[1]湖北医科大学口腔医学院,武汉430070 [2]洛阳市第四人民医院

出　　处：《临床口腔医学杂志》2000年第2期74-76,共3页Journal of Clinical Stomatology

摘　　要：探讨口腔鳞癌 IV型胶原 (Col l V)和层粘连蛋白 (L N)的表达及其与临床病理参数的关系。方法　应用免疫组化 SABC法检测了 30例口腔鳞癌组织和 5例人正常口腔粘膜组 Col 和 L N的表达 ,并用病理图像分析软件定量分析了两者的表达程度 ;对 Col IV和 L N的表达与临床病理参数的关系进行了统计学分析。结果　Col IV和 L N的表达部位相同 ,主要分布于口腔粘膜和鳞癌组织基底膜 ;正常粘膜 Col IV和 L N表达完整连续 ,在鳞癌组织中两者有不同程度的破坏 ;图像定量分析表明 :Col IV和 L N的表达与病理分级和临床分期无关 ,而和是否有颈淋巴结转移关系密切。结论　口腔鳞癌中 Col IV和 L N的破坏可能是发生颈淋巴结转移的必要条件之一。Objective To investigate the expression of collagen IV and laminin in oral squamous eell carcinoma and its correlation with clinicopathological parameters. Methods Immunohistochemical SABC methods was used to detect expression of collagen IV and laminin in 30 oral squamous cell carcinoma and 5 normal oral mucosa, the level of expression was analyzed quantitatively by image analysis software; Relationship between expression of collagen IV and laminin and differentiation, clinical stage, metastasis to neck lymph nodes was examined with Student t test.Results Collagen IV and laminin had similar expression location, which distributed in basement membrane of normal and cancer tissue; Complete and continuous expression could be seen in normal mucous tissue, however, their expression was destructed in some extent in cancer tissue; Expression of collagen IV and laminin showed no correlation with differentiation and clinical stage of cancer, but close relationship between expression and metastasis to neck lymph nodes was found. Conclusion Destruction of collagen IV and laminin is probably necessary to metastasis of oral squamous cell carcinoma to neck lymph nodes.

关 键 词：口腔鳞癌 IV型胶原 层粘连蛋白 颈淋巴结转移 

分 类 号：R739.802[医药卫生—肿瘤] R73-37[医药卫生—临床医学]