壳聚糖脱乙酰度测定方法的比较  

Comparison of Determination Methods for Degree of Deacetylation of Chitosan

作　　者：唐秉华 

机构地区：[1]广东湛江健力源医疗用品有限公司,广东湛江524000

出　　处：《广东化工》2012年第18期41-42,共2页Guangdong Chemical Industry

摘　　要：比较并分析壳聚糖脱乙酰度的两种测定方法。通过一组正交试验来制备不同脱乙酰度的壳聚糖,同时采用碱量法和紫外分光光度法进行脱乙酰的测定。分别从测定的原理和影响因素进行比较分析并进行测定结果的t检验。碱量法操作简便,但干扰因素多,结果重现性差;紫外分光光度法干扰因素少,客观、准确,结果重现性好。两种方法测定的结果存在一定差异。Two determination methods for degree of deacetylation of chitosan were compared and analyzed. Chitosans with various degrees of deacetylation were prepared by designing with orthogonal test and their degrees of deacetylation were determined by alkalimetry and UV-spectrophotometry. The principle and the influential factors of determination were compared and the determination results were carried out with t-test. Alkalimetry is simple and easy to be performed yet with more interferential factors and bad reproducibility. UV-spectrophotometry is proved to be simple, impersonal and accurate. The difference between results determined with two methods is significant.

关 键 词：壳聚糖 脱乙酰度 碱量法 紫外分光光度法 

分 类 号：O636.1[理学—高分子化学]