9.1C3分子抑制杀伤性细胞的细胞毒作用及其机制  

The inhibitory effect of 9.1C3 molecule on cytotoxicity of human killer cells

作　　者：欧阳为明[1] 金伯泉[1] 李琦[1] 李林[1] 刘雪松[1] 韩卫宁[1] 李德敏[1] 夏海滨[1] 

机构地区：[1]西安第四军医大学免疫教研室,西安710032

出　　处：《中国免疫学杂志》2000年第5期235-237,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目! (NO .39770 381)

摘　　要：探讨 9 1C3分子对杀伤细胞功能的影响。方法 :以新鲜分离的PBMC或混合淋巴细胞培养活化的淋巴细胞为效应细胞 ,采用重导向杀伤实验 (Redirectedkillingassay ,RKA)观察 9 1C3抗体对效应细胞杀伤P815细胞作用及其杀伤过程中TNF 浕分泌的影响?峁?:9 1C3抗体能显著抑制杀伤细胞对靶细胞的细胞毒作用 ,并使效应细胞分泌TNF 浕水平降低。结论 :9 1C3分子是一种抑制型杀伤细胞受体。Objective:To investigate the effect of 9.1C3 antibody on human killer cells.Methods:Redirected killing assay was employed to study the effect of 9.1C3 antibody on cytotoxic activity of killer cells, in which freshly isolated PBMC or mixture lymphocyte culture cells were used as effectors,and P815 was used as target cells. Results:Cytotoxicity of effectors induced by CD16 was significantly inhibited by 9.1C3 antibody and the secrection of TNF-ɑ was also inhibited during the killing phase.Conclusion:9.1C3 molecule is a kind of killing cell inhibitory receptor.

关 键 词：细胞毒 TNFΑ 抑制型杀伤细胞受体 9.1C3分子 

分 类 号：R392.12[医药卫生—免疫学]