乳铁蛋白对小鼠的辐射防护作用  

The Radioprotective Effect of Human Lactoferrin in Mice

作　　者：封丽[1] 李福生[1] 魏超[1] 张远[1] 乔建伟[1] 邵传静 

机构地区：[1]山东省医学科学院放射医学研究所 [2]济南市历下区疾病预防控制中心

出　　处：《中国辐射卫生》2012年第4期399-400,共2页Chinese Journal of Radiological Health

基　　金：山东省科技厅攻关项目(2010GSF10251)

摘　　要：目的研究乳铁蛋白对辐射损伤小鼠的保护作用。方法小鼠随机分成3组,空白对照组、单纯照射组、给药组。给予6.8Gy照射剂量小鼠30d观察各组存活率实验;给予2Gy照射剂量观察各组小鼠的骨髓微核率并对有核细胞进行计数。结果单纯照射组小鼠照后死亡率较高,25d全部死亡,与给药组比较存活时间差异有统计学意义(P<0.05),一般情况也有统计学意义(P<0.05)。单纯照射组照后小鼠骨髓有核细胞数明显下降,而乳铁蛋白给药组(包括照前给药和照后给药)高于单纯照射组,比较差异有统计学意义(P<0.05)。单纯照射组照后3d小鼠骨髓的微核细胞率明显升高,而乳铁蛋白给药组低于单纯照射组,比较差异有统计学意义(P<0.05)。结论乳铁蛋白无论是照前给药还是照后给药都具有较好的抗辐射作用。Objective To study the effect of human lactofcrrin on the X - ray Radiation Injury in mice. Methods The mice were randomly divided into three groups：control group,radiation group and llactoferrin group. To give mice 6.8 Gy, observe mice＇ 30 d survival rate and to give mice 2 Gy, observe bone marrow micronucleus, nuclear cell count. Results All the mice of radiation group without Lactoferrin died in 25d after exposure. The group the 30 - day livability is high, there was a significant difference in comparison between radiatin group without lactoferrin and groups with lactoferrin （ P 〈 0.05 ）. In the group without lactoferrin , the bone marrow karyocytes decreased significantly after radiation, and If treatment groups （ including prevention and emergency） arc higher than pure radiation group, there was a significant difference in comparison between radiatin group without lactoferrin and groups with lactoferrin （ P 〈 0.05 ）. Simply after radiation for 3 days, mice micronucleus bone marrow cells shouwed a significantly higher rate, and the group without lactoferrin, it shouwed less than the group with lactoferrin , with significant difference （ P 〈 0.05 ）. Conclusion lactoferrin which is used in the prevention iron or emergency to has radioprotective effect.

关 键 词：乳铁蛋白 辐射损伤 抗辐射效果 生存率 微核 

分 类 号：Q691[生物学—生物物理学]