龙眼保鲜功能微生物FJAT-0809-GLX(Brevibacillus brevis)功能成分的分析  

Functional Components in Brevibacillus brevis FJAT-0809-GLX Determined by GC/MSD

作　　者：车建美[1] 陈峥[1] 史怀[1] 刘波[1] 

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003

出　　处：《福建农业学报》2012年第10期1106-1111,共6页Fujian Journal of Agricultural Sciences

基　　金：中国热带农业科学院中央级公益性科研院所基本科研专项(1630042012006);农业部引进国际先进农业科学技术重点项目(2011-G25);福建省财政专项-福建省农业科学院科技创新团队建设基金(STIF-Y03);农业科技成果转化资金项目(2012GB2C400218);福建省农业科学院科技下乡"双百"行动项目(sbmn1211)

摘　　要：从保鲜功能微生物短短芽胞杆菌FJAT-0809-GLX(Brevibacillus brevis FJAT-0809-GLX)乙醇提取物功能成分中共鉴定出24种化合物,其中12种为匹配度在90%以上的高匹配度成分,相对含量较高的2种成分,分别为羟苯乙酯(24.28%)和邻苯二甲酸单(2-乙基己基)酯(19.78%),这2种物质成分相对含量总和占所有成分的44.06%,很有可能是其主要活性成分。保鲜功能微生物功能成分对大肠杆菌K88具有一定的抑菌效果,其中,羟苯乙酯的抑菌圈直径为17.81mm。同时对龙眼果皮过氧化物酶活性也具有一定的抑制效果。保鲜功能微生物功能成分按其功能可以划分为以下2种类型,一种为具有防腐杀菌(或抑菌)作用的物质,另一种为挥发性或者芳香类物质。Functional components in the ethanol extraction of Brevibacillus brevis FJAT-0809-GLX were determined by means of GC/MSD.Twenty four compounds were identified.Among them,12 displayed over 90% match with their respective standards.The relative contents of ethylparaben and 1,2-benzenedicarboxylic acid,mono（2-ethylhexyl）ester were 24.28% and 19.78%,respectively.Their total contents were significantly higher than that of other substances other substances found in the analysis.These two compounds were speculated to be associated with the antibacterial activity of Brevibacillus brevis FJAT-0809-GLX.The ethanol extraction of B.brevis FJAT-0809-GLX had an inhibitory effect on Escherichia coli K88,among them,the inhibition zone diameter of ethylparaben was 17.81 mm.It was also found to inhibit the activity of peroxidase（POD）.According to their functions,the identified substances were classified as either antibacterial or volatile flavor compounds.

关 键 词：短短芽胞杆菌 功能成分 GC MSD 

分 类 号：S646[农业科学—蔬菜学]