调节性T细胞对肿瘤特异性CTL杀伤效能的影响  

Effects of regulatory T cell on the kill activity of tumor-specific cytotoxicity of CTL

作　　者：张燕[1] 李红岩[1] 

机构地区：[1]河北医科大学第三医院肛肠外科,石家庄市050051

出　　处：《河北医药》2012年第24期3696-3698,共3页Hebei Medical Journal

基　　金：河北省医学科学重点课题计划资助项目(编号:08120)

摘　　要：目的探讨CD+4CD+25Treg细胞对肿瘤特异性CTL杀伤效果的影响及机制。方法将C57BL/6小鼠80只随机分为4组,每组20只。实验组1:DC与T细胞共同培养前删除CD+4CD+25Treg;实验组2:DC与T细胞共同培养后删除CD+4CD+25Treg;实验组3:DC与T细胞共同培养时不删除CD+4CD+25Treg;对照组:无DC诱导的T细胞。应用脾脏来源DC细胞诱导T细胞制备CTL,在CTL形成的不同时期采用MACS法删除CD+4CD+25Treg。应用MTT法检测不同组别CTL对B16黑色素瘤细胞的杀伤效果。同时应用ELISA法检测细胞培养液中IL-2、IL-10含量变化。结果 3实验组CTL杀伤率明显高于对照组(P<0.05)。实验组1及实验组2删除CD+4CD+25Treg的CTL杀伤率明显高于未删除的实验组3(P<0.05)。但CTL形成的不同时期删除CD+4CD+25Treg对CTL杀伤率无显著差别(P>0.05)。结论删除CD+4CD+25Treg细胞可明显提高CTL的杀伤效果,是打破肿瘤免疫耐受机制的新途径。Objective To explore the effects and the mechanism of CIM ＋ CD25 ＋ Treg on tumor-specific cytotoxicity of CTL to B16 Melanoma. Methods Eighty C57BL/6 mice were divided into three experimental and one control groups with 20 mice in each group. Mononuclear cells were isolated from spleen tissues of the mice, and CTLs were prepared by using DCs loaded with B16 tumor antigens. Tumor inhibition rate was evaluated by using MTr assay in vitro after deletion of CD4 ＋ CD25 ＋ Treg by using MACS, and the contents change of IL-2 and IL-10 in cell culture fluid were measured by using ELISA. Results Tumor inhibition rates in three experiment groups were significantly higher than that in control group（ P 〈 0.05 ）. The tumor inhibition rate in group 1 and 2 with a deletion of CD4 ＋ CD25 ＋ Tregs were significantly higher than that in group 3 with no deletion of Tregs（ P 〈 0.05 ）. But no significant difference were found between these two groups with different deletion time of Tregs while culturing （ P 〉 0.05 ）. Conclusion The deletion of CD4 ＋ CD25 ＋ Treg can significantly improve the tumor inhibition efficiency of antigen specific CTLs and It will be a new way to breakdown the tumor immune tolerance.

关 键 词：CD4+CD25+调节性T细胞 树突细胞 细胞毒T细胞 白介素-2 白介素-10 免疫耐受 

分 类 号：R730.5[医药卫生—肿瘤]