山东地方鸡种Mx基因遗传变异与免疫性状的关系  

Associations between immune traits and genetic variation of Mx gene in Shandong indigenous chicken populations

作　　者：王世波[1,2,3] 雷秋霞[2,3] 李福伟[2,3] 韩海霞[2,3] 周艳[2,3] 李桂明[2,3] 曹顶国[2,3] 单虎[1] 

机构地区：[1]青岛农业大学动物科技学院,山东城阳266109 [2]山东省农业科学院家禽研究所,山东济南250023 [3]山东省家禽育种工程技术研究中心,山东济南250023

出　　处：《浙江农业学报》2012年第6期969-974,共6页Acta Agriculturae Zhejiangensis

基　　金：山东省自然基金资助(ZR2009DM004);山东省良种工程高新育种子课题(2008LZ015);山东地方鸡种质资源评价及创新利用(2012GZX21027)

摘　　要：采用PCR-SSCP技术研究山东地方鸡种(鲁禽鸡、琅琊鸡、石歧杂鸡、汶上芦花鸡、莱芜黑鸡、济宁百日鸡)Mx基因多态位点与绵羊红细胞(sheep red blood cell,SRBC)免疫抗体滴度和禽流感(avian influenza,AI)、新城疫(newcastle disease,ND)免疫抗体滴度等免疫性状的关系。扩增包含Mx基因intron 13和exon 14约150 bp的片段,通过SSCP分型、测序,在5个地方鸡种共发现2个SNP突变位点分别为Mx基因编码序列第1 892位点G→A(Ser→Asn),第1 911位点G→A。与免疫性状进行关联分析,发现鲁禽鸡、琅琊鸡、莱芜黑鸡Mx基因编码序列1 892位点与H9抗体滴度显著相关(P<0.05)。The PCR-SSCP was used to study the associations between immune traits（ sheep red blood cell, avian in- fluenza and newcastle disease）and polymorphic loci of the Mx gene in Shandong indigenous chicken populations （Luqin chicken, Shi qiza chicken, Langya chicken, Laiwu black chicken and Jining Baifi chicken）. The fragment of Mx gene about 150 bp including intron 13 and exon 14 was amplified. The results showed that two single nueleotide poly- morphisms were detected in the five indigenous chicken populations by PCR-SSCP： 1 892 points of coding sequence of Mx gene G→A （Ser→Asn） ; 1911 points G→A, which couldn＇t lead to amino acid mutation. Through the analy- sis on the association between the polymorphic loci of the exon 14 and the antibody response, the SNPs on the 1892 points of coding sequence of Mx gene in Luqin chicken, Langya chicken and Laiwu black chicken was correiated to antibody response of H9 significantly（ P 〈 0. 05 ）.

关 键 词：鸡 MX基因 PCR—SSCP 免疫性状 

分 类 号：S858.31[农业科学—临床兽医学]