褪黑素对谷氨酸所致动物脑内MDA生成的抑制作用  

The Inhibitory Effect of Melatonin on the Level of MDA in the Animal′s Brain Induced by L Glutamate

作　　者：杨红英[1] 李经才[1] 刘玉娟 付爱玲 张若明[1] 

机构地区：[1]沈阳药科大学药学系,沈阳110015

出　　处：《沈阳药科大学学报》2000年第3期200-202,共3页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目 !编号 :395 70 82 0

摘　　要：用体内及体外两种实验方法 ,研究了谷氨酸对鼠脑组织中过氧化脂质及还原型谷胱甘肽含量的影响 ,以及施用褪黑素 (MT)后对谷氨酸的对抗作用 .结果表明 ,在体外 ,不同浓度的谷氨酸(2 9～ 11 7mmol/L )均能显著提高大鼠脑匀浆中丙二醛 (MDA )的含量 ;而加入MT(1～ 10mmol/L) ,可显著的对抗这种升高 .体内实验 ,小鼠侧脑室给予不同浓度的谷氨酸可显著升高全脑组织中丙二醛 (MDA)的含量及降低还原型谷胱甘肽的含量 ;如预先腹腔注射MT(1～ 10mg·kg-1) ,可显著对抗谷氨酸的影响 .MT的这种作用可能是其抗衰老的机制之一 .The level of MDA in the brain induced by L glutamate and the inhibitory effect of melatonin were studied in vitro and in vivo. The results indicated that, in vitro ,the L glutamate of different concentrations(2 9～11 7 mmol·L -1 )was capable of remarkedly increasing and MT(1～10 mmol·L -1 ) added together with L glutamate was capable of remarkedly reducing the level of MDA in homogenates of the rat′s whole brain; in vivo ,the L glutamate of different doses(50～200 μg·mice -1 )were injected(i.c.v.)into the mice,then the level of MDA was increased and the level of GSH was decreased,and MT i.p.1～10 mg·kg -1 day 1 for 7 days was capable of reducing the level of MDA and increasing the level of GSH.It is considered that this effect of MT is likely to be one of its anti aging mechanisms.

关 键 词：褪黑素 谷氨酸 自由基 衰老 动物实验 

分 类 号：R339.38[医药卫生—人体生理学] R977.4[医药卫生—基础医学]