山楂提取物对脂肪酸合酶的体外抑制作用  

Inhibitory effect of extract of Hawthorn on fatty acid synthetase in vitro

作　　者：王鹏[1] 高敏艳[1] 高岚[1] 肖学凤[1] 

机构地区：[1]天津中医药大学,300193

出　　处：《天津中医药大学学报》2012年第4期229-232,共4页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家自然基金资助项目(30973932)

摘　　要：[目的]研究山楂提取物对脂肪酸合酶(FAS)的体外抑制作用,并初步探索抑制作用机制。[方法]采用紫外—分光光度法,通过监测340 nm下还原型辅酶Ⅱ(NADPH)吸光度值的变化,测定FAS全反应及不同反应中心的活性,研究不同剂量山楂醇提物和水提物对FAS体外抑制作用。[结果]山楂水提物给药浓度为100μg/mL时,酶剩余活性为34.2%,醇提物给药浓度达到150μg/mL时,仍剩余64.2%的酶活性。两者都作用于FAS结构域中的酮酰还原域(KR)和烯酰还原域(ER),并且这两个结构域是山楂醇提物抑制FAS的重要作用位点。[结论]山楂提取物对FAS存在抑制作用。[Objective] To study the inhibitory effect of extract of Hawthorn on fatty acid synthetase （FAS） in vitro and explore its mechanism. [Methods] After treatment with different doses of ethanol and water, in these extracts of Hawthorn, the residual activity of FAS as overall reaction and different active sites were separately detected at 340 nm in ultraviolet-spectrophotometer through monitoring the alteration of OD value of NADPH. [Results] After treatment with aqueous extract（100 μg/mL）, the residual activity of FAS was 34.2%. And after treatment with ethanol extract of higher concentration （150 μg/mL）,the residual activity of FAS was 64.2%. Both of them can inhibit the Keto-acyl reduction and Enoyl reduction,and the two active sites were important for ethanol extract of Hawthorn. [Conclusions] FAS can be inhibited by the extract of Hawthorn.

关 键 词：脂肪酸合酶 山楂 醇提物 水提物 抑制作用 抑制机制 

分 类 号：R285.5[医药卫生—中药学]