卷丹百合辐射诱变的生物学效应及变异研究初报  

Preliminary studies on biological effects of Lilium lancifolium radiation mutagenesis and its variation

作　　者：朱校奇[1] 周佳民[1] 黄艳宁[1] 鲁耀雄[1] 李文革[2] 罗志平[2] 张天术[3] 

机构地区：[1]湖南省农业科学院农业生物资源利用研究所,长沙410125 [2]湖南省农业科学院核农学与航天育种研究所,长沙410125 [3]龙山县农业技术推广中心,湖南龙山422200

出　　处：《南方农业学报》2012年第11期1638-1641,共4页Journal of Southern Agriculture

基　　金：湖南省科技支撑计划项目(2010NK3079);湖南省农业科学院科技创新项目(2009HNNKYCX16)

摘　　要：【目的】探索药食兼用型卷丹百合辐射诱变的生物学效应、变异特点。【方法】以湖南龙山卷丹百合为材料,用0～3Gy4个不同剂量60Co-γ射线辐射处理其鳞茎,分析其辐射后代的性状表现及变异情况。【结果】经0、1、2、3Gy60Co-γ处理的成苗(株)率分别为100%、100%、60%和0。诱变卷丹百合第1营养世代(VM1)出现较明显的辐射损伤,其株高、茎粗、单株珠芽数、单株鳞茎鲜重均随着辐照剂量的增加而降低。第2营养世代(VM2)的株高、单株珠芽数和单株鳞茎重等性状出现分离,且三者的变异具有一致性,均以2Gy60Co-γ处理的变幅较大。【结论】用60Co-γ射线辐射诱变卷丹百合有明显效果,适宜诱变剂量为2Gy60Co-γ;选择获得1株优良卷丹百合变异株。Abstract： [Objective]This research explored the biological radiation effects, various characteristics, and mutagenic effect of medicinal and consumable dual-purpose Lilium lancifolium. [ Method ]Using Hunan Longshan Lilium lancifolium as test material, 4 different doses of 60Co-γradiation processing in 0-3 Gy were used to treat bulb and to analyze the radiation characteristics and variations of progeny. [Result]After the seedlings were treated with 0, 1, 2, and 3 60Co-γ, the seedling rates were 100, 100, 60, and 0%, respectively. Mutagenesis of the first vegetative generation Lilium lancifolium （VM1） appeared to be more visibly radiation-damaged; also, the plant height, stem diameter, builbil number per plant, and fresh bulb weight decreased as the irradiation dosage increased. The three consistent variations of the second vegetative generation Lilium lancifolium （VM2）, plant height, builbil number per plant, and bulb weight, displayed clear separations. By the 2 Gy 60Co-γ treatment, large variations amongst the characteristics were detected. [Conclusion]The appropriate dosage of ray was 2 Gy 60Co-γfor Lilium lancifolium bulb. Using 60Co-γ ray radiation mutagenesis displayed obvious effects on Lilium lancifolium, in which one of the superior Lilium lancifolium variant strains could he selected.

关 键 词：卷丹百合 60Co-γ 辐射诱变 生物学效应 变异 

分 类 号：S682.29[农业科学—观赏园艺]