难治性根尖周炎根尖生物膜内粪肠球菌的检出及临床意义  

Analysis of relationship between symptoms of the clinical patients and the detection of enterococcus faecalis in extraradicular biofilm of refractory periapical periodontitis

作　　者：孟磊[1] 卢志山[1] 柳忠豪[1] 张韬文[1] 任光辉[1] 

机构地区：[1]滨州医学院附属烟台市口腔医院,264008

出　　处：《中华口腔医学研究杂志（电子版）》2012年第6期28-31,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：烟台市科学技术发展计划(2007141)

摘　　要：目的探讨难治性根尖周炎根尖生物膜内的粪肠球菌的检出率,分析粪肠球菌的检出率与临床表现的关系。方法按纳入标准收集需要进行根尖外科手术的单根管患牙42颗,记录症状和体征,采集根尖生物膜样本,各样本均采用生化鉴定和聚合酶链反应(PCR)两种方法检测粪肠球菌。统计学分析两种方法对粪肠球菌的检出率差异及其与患者症状体征之间的关系。结果生化鉴定和PCR检测难治性根尖周炎根尖生物膜内粪肠球菌的检出率分别为52.4%和71.4%,差异有统计学意义(P<0.05)。PCR检测发现在疼痛组粪肠球菌的检出率高于无疼痛组(P<0.05)。结论 PCR检测难治性根尖周炎根尖生物膜内粪肠球菌检出率较高,且粪肠球菌的检出与疼痛存在相关性。Objective Identification and detection of Enterococcus faecolis （E.faecalis） isolated from extraradicular biofilm in refractory periapical periodontitis, in order to analyze the relationship between the occurrence of E.faecalis and clinical symptom. Methods Forty-two single root teeth which need periapical surgery were collected, and the clinical symptoms and physical signs were recorded. Bacterium samples from extraradicular biofilm were taken. The occurrence of E.faecalis by means of culture and polymerase chain reaction was investigated. The presence of E.faecalis and clinical signs was assessed to investigate the potential relationship between clinical findings. Results Culture and PCR revealed that the detection rates of E.faecalis was 52.4% and 71.4%, respectively, there was statistical significance （P 〈 0.05）; The detection rates of E.faecalis by means of PCR between the pain and the painless group showed statistical significance （P 〈 0.05）. Conclusions PCR was sensitive in detecting E.faecalis in root apex of refractory periapical periodontitis. The occurrence of E.faecalis in cases of refractory periapical periodontitis correlates with pain.

关 键 词：难治性根尖周炎 根尖生物膜 粪肠球菌 

分 类 号：R781.34[医药卫生—口腔医学]