新基因Nischarin生物信息学分析及初步验证  

Initial Bioinformatics Analysis and Verification of a Novel Gene Named Nischarin

作　　者：赵太云[1] 王勃[1] 苏瑞斌[1] 吴宁[1] 李锦[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《现代生物医学进展》2012年第32期6201-6206,共6页Progress in Modern Biomedicine

基　　金：国家自然基金重点项目(30930040);国家自然基金青年项目(30701016;81102426);北京市自然基金面上项目(7082073)

摘　　要：目的:对新基因Nischarin进行生物信息学分析,探索其新功能特征,并通过实验进行初步验证。方法:用生物信息学方法对Nischarin进行初步分析,阐明了它的基因结构、染色体定位、编码蛋白质的理化性质、相互作用基因、相互作用蛋白、亚细胞定位、蛋白质功能域等信息。最后采用细胞免疫荧光对其DNA结合位点进行初步验证。结果:对新基因Nischarin的上述性质进行了有效的预测,分析表明该基因结构复杂,相互作用基因或蛋白多,亚细胞分布预测复杂。验证了Nishcarin存在的DNA结合位点。结论:通过生物信息学分析,表明新基因Nischarin是一个复杂的基因,可能存在的多种蛋白表达形式、这些不同的蛋白可能存在不同的亚细胞分布,且该蛋白可能与多种蛋白存在相互作用,上述基因和蛋白特性可能是Ⅰ型咪唑啉受体(Imidazoline-1 receptor,I1R)复杂药理学作用的分子基础。Objective： To explore the new function of the novel gene named Nischarin and verify the real nature, the gene was an-alyzed by the bioinformatics analysis. Methods： Initial bioinformatics analysis was performed on the novel gene named Nischarin. Its gene structure, genome localization, the physical and chemical characteristics of the protein, subcellular localization of the protein, func- tional domain and so on was predicted. On the basis of the predicted result, the DNA binding motifs （Leucine zipper pattern） was verified by cell immunofluorescence. Results： Through efficient bioinformatics analysis, Nischafin gene＇s structure is very complex. It may be ex- pressed by many kinds of proteins. The proteins have no signal peptide and it＇s subcellular localization is complex. There may be a DNA binding motifs in the Nischarin protein. Conclusion： Nischarin is a very complex gene, it may be expressed many kinds of proteins which maybe have different subcellular localization. Nischarin protein has interaction with many proteins. This may be the molecular basis for I1R complex pharmacology.

关 键 词：NISCHARIN 生物信息学 Ⅰ型咪唑啉受体 可变剪接异构体 

分 类 号：Q-33[生物学] Q75