玉米胚SOD提取物对糖尿病大鼠血糖、血脂及血清氧化应激状态的影响  

Effect of Corn Embryo SOD Extracts on Glucose Levels, Serum Total Cholesterol, Triglyceride and Oxidative Stress in Blood

作　　者：于德泉 逯晓波[2] 刘秋芳[2] 靳翠红[2] 宋岩松[3] 商冠宁 

机构地区：[1]辽宁省肿瘤研究所,辽宁沈阳110042 [2]中国医科大学公共卫生学院卫生毒理教研室,辽宁沈阳110001 [3]中国医科大学高职学院,辽宁沈阳110001

出　　处：《现代生物医学进展》2012年第33期6422-6425,共4页Progress in Modern Biomedicine

基　　金：辽宁省教育厅科学技术项目(L2010703)

摘　　要：目的:探讨玉米胚SOD提取物对于糖尿病大鼠血糖及主要脏器抗氧化能力的影响。方法:成年雄性Wistar大鼠36只,随机分为以下6组:1)正常对照组;2)糖尿病模型组:腹腔注射四氧嘧啶175mg/kg,连续两天;3)二甲基双胍对照组:糖尿病模型+灌服10mg/kg盐酸二甲基双胍;4)SOD高剂量组:糖尿病模型+灌服0.3 g SOD提取物;5)SOD中剂量组:糖尿病模型+灌服0.18g SOD提取物;6)SOD低剂量组:糖尿病模型+灌服0.06 g SOD提取物。连续3周,动态监测血糖,血清总胆固醇和甘油三酯。45天后处死动物,提取血清,采用硫代巴比妥酸法测定脂质过氧化产物丙二醛(MDA)含量;DTNB法测定还原型谷胱甘肽(GSH)含量;黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活力。结果:不同剂量的玉米胚SOD提取物对四氧嘧啶所致高血糖模型大鼠具有明显的降低血糖作用,可降低血清中总胆固醇和甘油三酯。并使血清MDA水平降低,抗氧化物质GSH含量增加,SOD活力升高。结论:玉米胚SOD提取物具有一定的降低血糖、血脂功效,可能与增加机体抗氧化能力有关。Objective： To investigate the effect of corn embryo SOD extracts on glucose levels and anti-oxidative function in blood, liver, kidney and brain of diabetic rats. Methods： 36 male Wistar rats were randomly divided into six groups, 1） normal control group; 2） diabetic model group （175mg/kg bw tetraoxypyrimidine injection）; 3） diabetic model group （diabetic model ＋ 10mg/kg metformin）. 4） high SOD group（diabetic model＋0.3g/kg bw SOD feeding）; 5） middle SOD group; （diabetic model＋0.18g/kg bw SOD）; 6） （diabetic model＋0.06g/kg bw SOD）. The rats were perfused a stomach with corn embryo SOD extracts for 4 weeks continuously and sacrificed animals after 45 days and then the blood taken. MDA level were measured by TBA method, GSH level activity were measured by modified DNTB method. SOD activities were measured by Xanthine oxidase methods. Results： Compared with that in the diabetic model group, glucose and MDA levels in SOD group decreased, and GSH content and SOD decreased. Conclusions： Corn embryo SOD extracts can decrease glucose level serum total cholesterol and triglyceride of the diabetic rats＇ and its mechanism is related to anti-oxidative function.

关 键 词：血糖 血脂 玉米胚 糖尿病 氧化应激 

分 类 号：Q95-3[生物学—动物学] R587.1[医药卫生—内分泌]