一个自闭症家系的B淋巴细胞周期研究  

Altered Cell Cycle of B Lymphocytes from an Autistic Family

作　　者：申晨[1] 邹小兵[2] 邹俊华[3] 申阿东[1] 钟南[3] 

机构地区：[1]首都医科大学附属北京儿童医院儿科研究所,北京100045 [2]中山大学附属第三医院儿童发育行为中心,广东广州510630 [3]北京大学医学部医学遗传学系,北京100083

出　　处：《标记免疫分析与临床》2012年第6期356-359,共4页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金(No.2007CB511902)

摘　　要：目的了解自闭症儿童的B淋巴细胞的细胞周期是否存在变异,为寻找自闭症的生物标志物并进一步为揭示自闭症的发病机制奠定基础。方法运用B淋巴细胞建系、淋巴细胞培养、细胞周期检测以及统计学分析的方法,对一个汉族自闭症家系进行研究。该家系由父母及6个子女组成,父母表现型正常,而其中3名子女为自闭症患儿,3名为非自闭症儿童。结果分析显示,B淋巴细胞的细胞周期在自闭症儿童组(相对于非自闭症儿童组)出现了S期细胞比例的增加和G0/G1期细胞比例的减少,同时代表增殖能力的"S期+G2/M期"的细胞比例也出现显著增加。结论细胞周期的变化依赖于细胞内调控机制的改变,可能涉及到多种细胞周期蛋白的表达和调控变化,自闭症患儿存在B淋巴细胞细胞周期变异这一现象为进一步揭示自闭症的发病机制提供了线索。此外,如果本研究结果能够在大样本中得到肯定,则可作为自闭症的生物标志物用于疾病辅助诊断而具有良好的临床应用价值。Objective To discover whether the cell cycle of B lymphocyte from autistic children were different from inartistic children. Methods One family including parents, three autistic children and three non-autistic children was involved in this study. The B lymphoblastoma cell construction, cell culture and cell cycle detec- tion by flow cytometly （FCM） were carried out. Results FCM analysis showed that the percentage of G0/G1 phase cells decreased but that of S phase cells increased in autistic children compared with non-autistic sib- lings. The cell percentage of ＂S ＋ G2/M＂ phase which represents cell proliferation also increased significantly in autistic children. Conclusion Alteration of cell cycle is the result of the intracellular regulating, which may involve variety changes of protein expression and regulation. The phenomenon that autistic children had alerted B lymphocyte cell cycle gives us a clue to further reveal the pathogenesis of autism. In addition, if the result of this study can be confirmed in a large sample further, it could be used as a biomarker for autism diagnosis and may have a good clinical value.

关 键 词：自闭症 B淋巴细胞 细胞周期 

分 类 号：R446.6[医药卫生—诊断学] R749.94[医药卫生—临床医学]