STRtyper-10F/G联合CODIS系统鉴定突变三联体和二联体  

The evaluation of STRtyper-10F/G^(TM) and CODIS in triplet cases with inconsistent-loci and duo-paternity identification cases

作　　者：张艳萍[1] 王琳[1,2] 王毅[1] 王晓梅[1] 陈先丽[1] 程捷[1] 刘庆[1] 马旭[1,2] 

机构地区：[1]国家人口计生委科学技术研究所,北京100081 [2]中国协和医科大学,北京100730

出　　处：《中国法医学杂志》2012年第6期445-447,共3页Chinese Journal of Forensic Medicine

摘　　要：目的评估STRtyper-10F/G、CODIS系统联合应用在突变三联体和二联体亲子鉴定中的鉴定能力。方法104例亲子鉴定样本,采用CODIS检测,其中3例三联体发现突变的案例以及101例二联体中73例认定和28例排除案例,分别再使用STRtyper-10F/G检测;统计认定和排除情况、比较单独使用和联合使用在排除关系案例中的表现和法医学参数(H、DP、PE、PIC、TPI)。结果 3例存在突变基因的三联体案例加做STRtyper-10F/G未发现更多的矛盾基因座,PI值均大于10 000,可作出认定结论;73例二联体认定案例中13例PI不足10 000,加做STRtyper-10F/G系统后PI>10 000,可确认认定;28例排除案例最高排除率CODIS为50.00%、STRtyper-10F/G为64.29%;两个体系均具有较高的杂合度(H≥0.7)和信息量(PIC>0.7),联合应用CEP为0.999 999 999 505 3。结论 STRtyper-10F/G和CODIS系统联合应用可满足突变三联体和二联体亲子鉴定的需要。Objective To evaluate CODIS （combined DNA index system, the application value of the STR typer-lOF/GTM combined with CODIS） in triplet cases with inconsistent-loci and duo-paternity identification cases. Methods 3 cases of identification were detected by using STRtype triplet with inconsistent-loci and 101 cases of duo-paternity r-lOF/GTM and CODIS. The paternity inclusion and exclusion of the STRtyper-10F/GTM and COD1S were analysed. Separate system and joint systems were compared on forensic parameters （H, DP, PE, PIC, TPI） and exclusive cases. Results After tested by STRtyper-10F/GTM, no inconsistent-loci were further found in 3 triplet cases with inconsistent-loci, and all of the paternity Index （PI） were above 10 000. The inclusion of paternity could be made. When detected only by CODIS, the PI of 13 duo-paternity cases was less than 10 000. After combined with the STRtyper-10F/GTM, all of their PI were above 10 000, and the inclusion of paternity could be made. For the 28 exclsive cases, the highest exclusion rates of the CODIS and STRtyper-10F/G were 50. 00% and 64. 29% respectively. Both systems had high heterozygosity （ H ≥ 0. 7） and polymorphic information content （ PIC 〉 0. 7 ）, and the cumulative probability of exclusion （CEP） of the STR typer-10F/GTM combined with CODIS was O. 999 999 999 505 3. Conclusion The STRtyper-lOF/GTM and CODIS systems are powerful and reliable for triplet cases with inconsistent-loci and duo-paternity identification cases.

关 键 词：法医遗传学 亲子鉴定 短串联重复序列 STRtyper-10F GTM CODIS 

分 类 号：D919[医药卫生—法医学]