二甲双胍对急性早幼粒细胞白血病细胞系NB4的作用及机制探讨  

Effect of Metformin on Acute Promyelocytic Leukemia Cell Line NB4 and its Mechanism

作　　者：怀磊 王翠翠 张翠萍 李其辉 陈一瑞 贾玉娇 王敏[1] 王建祥 

机构地区：[1]中国医学科学院、北京协和医学院血液学研究所、血液病医院实验血液学国家重点实验室,天津300020

出　　处：《中国实验血液学杂志》2012年第6期1322-1326,共5页Journal of Experimental Hematology

基　　金：国家自然基金(编号30971290);天津市应用基础研究计划重点项目(编号10JCZDJC19600)

摘　　要：本研究旨在探讨二甲双胍对急性早幼粒细胞白血病细胞系NB4细胞生物学特性的影响及可能的作用机制。采用不同浓度的二甲双胍处理NB4细胞,MTT法检测细胞增殖情况,流式细胞术检测细胞凋亡情况,通过细胞黏附实验分析药物对细胞黏附能力的影响。采用细胞外信号调节激酶(ERK)磷酸化抑制剂U0126预处理NB4细胞,Western blot观察ERK磷酸化水平的变化,同时检测细胞凋亡和黏附能力的改变。结果表明,二甲双胍能抑制NB4细胞增殖,诱导细胞凋亡,增强细胞的黏附能力。5μmol/L U0126预处理有效抑制NB4细胞中ERK的磷酸化,降低5 mmol/L二甲双胍诱导的细胞凋亡并减弱细胞的黏附能力。结论:二甲双胍对NB4细胞有抑制增殖、促进凋亡和黏附的作用,MEK/ERK信号通路可能是二甲双胍在NB4细胞中的分子作用机制之一。The purpose of this study was to investigate the effect and molecular mechanism of metformin （Met） on biological characteristics of acute promyelocytic leukemia （APL） cell line NB4. NB4 cells were treated with various concentrations of Met for different time, MTr method was used to detect cell proliferation, the alteration of cell apoptosis was analyzed by flow cytometry, and the change of cell adhesion ability was examined by cell adhesion assay. NB4 cells were pretreated with U0126, a specific inhibitor for extracellular signal-regulated kinase （ERK） phosphorylation, ERK phosphorylation was assessed by Western blot analysis, apoptosis and cell adhesion ability were evaluated by flow cytometry and cell adhesion test respectively. The results showed that Met could inhibit the cell proliferation, induce the cell apoptosis and increase the ability of cell adhesion. The pretreatment of NB4 cells with 5 μmol/L U0126 could effectively inhibit the phosphorylation of ERK, and reduce cell apoptosis and adhesion induced by 5 mmol/L Met. It is concluded that Met can inhibit the proliferation and promote the apoptosis and adhesion of NB4 cells. MEK/ERK signaling pathway may be one of the molecular mechanisms of metformin on NB4 cells.

关 键 词：二甲双胍 急性早幼粒细胞白血病 NB4细胞系 MEK/ERK信号通路 

分 类 号：R733.71[医药卫生—肿瘤] R979.1[医药卫生—临床医学]