南瓜蛋白联合常用化疗药对NB4细胞增殖和凋亡的影响  

Effects of Cucurmosin Combined with Common Chemotherapeutics on Proliferation and Apoptosis of NB4 Cells

作　　者：谢捷明[1] 刘梅[2] 刘庭波[3] 陈明晃[4] 杨爱琴[1] 杨沛[3] 

机构地区：[1]福建医科大学药学院,福建福州350004 [2]陕西省榆林市第二医院血液科,陕西榆林719000 [3]福建医科大学附属协和医院血液病研究所,福建福州350001 [4]中国科学院福建物质结构研究所结构化学国家重点实验室,福建福州350002

出　　处：《中国实验血液学杂志》2012年第6期1327-1331,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(编号30772587);福建省自然科学基金资助项目(编号C0510012;2011J01188)

摘　　要：本研究旨在探讨南瓜蛋白(CUS)与全反式维甲酸(ATRA)或亚砷酸(ATO)联用对人急性早幼粒细胞白血病NB4细胞增殖和凋亡的影响。以MTT比色法检测CUS与ATRA或ATO联用对NB4细胞的增殖抑制作用,用流式细胞术检测CUS与ATRA或ATO联用对NB4细胞凋亡的影响,用金氏公式判断两药联用的协同效果。结果表明,CUS与ATRA或ATO联合应用的抑制率均大于各药相应浓度单用的抑制率,其协同指数q值均>0.85,并且两药均在低浓度范围内联用的协同效果明显。CUS与ATRA或ATO联合应用时NB4细胞的凋亡率均大于各药相应浓度单用时的凋亡率,其协同指数q值均>1.15。结论:CUS与ATRA或ATO联用对NB4细胞增殖和诱导凋亡均有较好的协同作用。This study was aimed to investigate the proliferation inhibition and apoptosis induction of cucurrnosin （CUS） combined with all trans-retinoic acid （ATRA） or arsenic trioxide （ATO） on human acute promyelocytic leukemia cell line NB4. MTT method was used to determine the proliferative inhibition of CUS combined with ATRA or ATO on NB4 cells, and flow cytometry was used to determine the apoptosis induction effect of CUS combined with ATRA or ATO on NB4 cells. Jin＇s formula was used to assess the synergistic effect of this combinations. The results showed that, compared with single drug, the proliferation inhibitory ratio and apoptotic ratio of CUS combined with ATRA or ATO on NB4 cells was higher than CUS, ATRA and ATO alone. The synergistrc index （q） were all larger than 0.85, and the combined effects were significant at low concentrations. It is concluded that the CUS combined with ATRA or ATO synergistically increases the effects of proliferative inhibition and apoptosis induction on NB4 cells.

关 键 词：南瓜蛋白 全反式维甲酸 亚砷酸 NB4细胞系 药物协同作用 

分 类 号：R733.71[医药卫生—肿瘤] R979.1[医药卫生—临床医学]