MPO、NQO1基因多态性与急性白血病易感性的相关研究  

Relationship of MPO and NQO1 Gene Polymorphisms with Susceptibility to Acute Leukemia

作　　者：贾明峰[1] 席亚明[1] 石秀娥[2] 张豪[1] 邓伟[1] 李明[1] 李培[1] 徐建旺[1] 马海珍[1] 姚小健[1] 

机构地区：[1]兰州大学第一医院血液科、血液病研究所,甘肃兰州730000 [2]甘肃省康复中心医院老年病科,甘肃兰州730000

出　　处：《中国实验血液学杂志》2012年第6期1336-1340,共5页Journal of Experimental Hematology

基　　金：甘肃省科技支撑项目(0708NKCA113);兰州大学医学科研基金资助项目(LZUYX2008)

摘　　要：本研究旨在探讨髓过氧化物酶(MPO)和醌氧化还原酶1(NQO1)基因多态性与中国甘肃人群急性白血病易感性的关系。用1∶1配对病例-对照研究和连接酶检测反应(LDR)分型方法分析急性白血病病例组(150例)和对照组(150例无癌住院患者)MPO和NQO1的基因多态性,比较不同基因型与急性白血病易感性的关系。结果表明,病例组MPO-463A等位基因分布频率低于对照组,MPO(G-463A)各基因型在病例组与对照组中的分布差异显著(χ2=11.828,P<0.05,OR=0.368,95%CI=0.205-0.610)。病例组NQO1-609T等位基因分布频率高于对照组,NQO1(C-609T)各基因型在病例组与对照组中的分布差异显著(χ2=17.931,P<0.05,OR=1.428,95%CI=1.237-3.339)。基因多态联合作用分析显示,MPO野生型兼具NQO1野生型者发生急性髓系白血病的风险降低至33.6%。结论:MPO、NQO1与急性白血病易感性相关,携带MPO(G-463A)突变基因型(GA/AA)可降低白血病的发病风险,携带NQO1(C-609T)突变基因型(TC/TT)可增加白血病的发病风险,MPO野生型与NQO1野生型者联合作用可进一步降低急性髓系白血病的发病风险。The aim of this study was to investigate the relationship of the gene polymorphisms of myeloperoxidase （MPO） and NAD （P）H：quinone oxidoreductase 1 （NQO1） with the susceptibility to acute leukemia（AL） in Chinese Gansu population. A 1：1 paired case-control study of 150 patients with acute leukemia and 150 cancer-free inpatients as a control was conducted to detect the polymorphisms of MPO and NQ01 by LDR techniques. The results showed that the MPO-463A genotype frequency in patient group was lower than that in control group, and there was significant difference of MPO （G-463A） genotype between patient group and control group （ X^2 = 11. 828, P 〈 0.05, OR = 0.368, 95 % CI = 0.205 -0.610）. The NQ01-609T genotype frequency in patient group was higher than that in control group, and there was significant difference of NQO1 （C-609T） genetype between patient group and control group （ X^2 = 17.931, P 〈 0.05, OR = 1. 428, 95% CI = 1. 237 - 3. 339）. The combined gene analysis showed that the AML risk in patients carry- ing the wild genotypes of MPO and NQ01 was dropped to 33.6%. It is concluded that the MPO and NQ01 gene poly- morphisms are associated with susceptibility to AL. The AL risk may decrease in patients carrying MPO （G-463A） mu- tant gene（ GA/AA）, while the AL risk may increase in patients carrying NQO1 （C-609T） mutant gene（TC/TT）. The combined effect of MPO and NQ01 wild genotypes may further decrease AL risk.

关 键 词：髓过氧化物酶 醌氧化还原酶 基因多态性 白血病易感性 

分 类 号：R733.71[医药卫生—肿瘤]