高渗盐水灭活自体骨再植的动物实验  

Animal experimentation of reimplantation of hypertonic saline-induced devitalized bone

作　　者：彭长亮[1] 杨毅[1] 孙馨[1] 郭卫[1] 

机构地区：[1]北京大学人民医院骨与软组织肿瘤治疗中心,北京100044

出　　处：《北京大学学报（医学版）》2012年第6期950-953,共4页Journal of Peking University:Health Sciences

摘　　要：目的:观察高渗盐水灭活自体骨的愈合过程和生物力学强度的变化,以评价自体骨高渗盐水灭活再植手术的效果,为临床应用提供基础理论依据。方法:建立新西兰大白兔尺骨段高渗盐水灭活模型,以自身对侧尺骨段生理盐水浸泡后原位再植作为对照,分别于3、6、12、24周行X线检查、三点弯曲负荷实验检查和HE染色组织学检查,对两组间生物力学数据进行t检验分析,P<0.05为差异有统计学意义。结果:X线片显示灭活骨于12周达到骨性愈合;观察组织切片,愈合过程中成骨细胞逐渐增多,分泌类骨质,新骨形成;最大破坏载荷逐渐升高,24周后达到最高[对照侧(206.25±16.64)N,实验侧(196.88±8.24)N,P>0.05]。结论:高渗盐水使灭活骨愈合过程减慢,但最终灭活骨通过膜内化骨和软骨内化骨达到愈合,其中以软骨内化骨为主,生物力学强度随时间的延长进行性的升高。Objective：To observe the healing process and the change of biomechanical properties of hypertonic saline-induced devitalized bone segment,so as to provide fundamental theory for clinical treatment.Methods：A model of New Zealand rabbit ulnar segments devitalized by hypertonic saline was established and then reimplanted in situ.The ulnar specimens were taken for examination of X-rays,light microscope and three-point-bend test at the end of 3,6,12,and 24 weeks postoperatively.Results：The devitalized bone healed at the end of 12 weeks in the X-ray film.The histological examination showed that osteoblast multiplied and secreted osteoid gradually.The maximal breaking load of the devitalized bone continuously increased and reached the top at the end of 24 weeks [control group（206.25±16.64） N vs.devitalized group（196.88±8.24） N,P0.05].Conclusion：The devitalized bone healed through intramembranous and endochondral ossification,and the endochondral ossification predominated;the biomechanical strength of devitalized bone continually increased as time lasted.

关 键 词：骨肿瘤 盐水 高渗 移植 自体 生物力学 动物实验 

分 类 号：R738.1[医药卫生—肿瘤]