MMP-9、PR、Ki-67和Survivin在多发性脑膜瘤中的表达  

Expression and significance of MMP-9, PR, Ki-67 and Survivin in multiple meningiomas

作　　者：宫杰[1] 朱树干[1] 吴承远[1] 李新钢[1] 刘玉光[1] 任晓辉[2] 张源[1] 

机构地区：[1]山东大学齐鲁医院神经外科,济南250012 [2]首都医科大学北京天坛医院,济南250012

出　　处：《中华医学杂志》2012年第45期3218-3220,共3页National Medical Journal of China

摘　　要：目的通过检测MMP-9、PR、Ki-67和Survivin在多发性脑膜瘤组织中的表达情况，探讨多发性脑膜瘤的发生、诊断及生物学特性。方法收集山东大学齐鲁医院1992至2005年手术治疗的多发性脑膜瘤36例，与30例单发性脑膜瘤进行对比研究，所有标本进行诊断、组织学分型和分级，以正常硬脑膜组织作对照，应用免疫组织化学技术检测MMP-9、PR、Ki-67、Survivin在脑膜瘤中的表达，并进行定量分析。结果MMP-9、PR、Ki-67、Survivin在正常硬脑膜组织中均未检测到，而在脑膜瘤表达率分别为100％（66／66）、53％（35／66）、23％（15／66）、88％（58／66），与正常组织比较差异有统计学意义，但多发性和单发性脑膜瘤中的表达阳性率差异无统计学意义。MMP-9、PR在两组的阳性表达强度差异有统计学意义，MMP-9在多发组中表达强度明显高于单发组（P〈0．01），而PR在多发组中表达强度明显低于单发组（P〈0．05），Ki-67、Survivin在两组中的阳性表达强度差异无统计学意义。结论MMP-9、PR、Ki-67、Survivin检测有助于脑膜瘤的早期临床诊断。Objective To detect the expressions of matrix metalloproteinase-9 （MMP-9）, progesterone receptor （PR）, Ki-67 and Survivin in the multiple meningiomas （MMs） and explore its genesis, diagnosis and bionomics features. Methods A total of 66 cases with histological sections of meningiomas retrieved from the archives of Department of Pathology of our hospital, including 30 cases of solitary meningiomas （SMs） and 36 cases MMs, were regrouped as benign, atypical and malignant by hematoxyhn and eosin staining according to the World Health Organization classification of nervous system tumors. Immunohistochemistry was performed to detect the expressions of MMP-9, PR, Ki-67 and Survivin in 36 cases of MMs and 30 cases of SMs. And normal brain tissue was selected as a control group. The staining intensity was analyzed quantitatively for the differential expressions of MMP-9, PR, Ki-67 and Survivin between SMs and MMs. Results No expression of MMP-9, PR, Ki-67 and Survivin was detected in 5 normal brain tissues, but the expression rates were 100%, 53%, 23% and 88% respectively for significant difference comparing with normal tissue. The result of statistical analysis showed that there was significant difference in the expression intensity of MMP-9 and PR between two groups. The expression intensity MMP-9 in multiple group was significantly higher in MMs than that in SMs （ P 〈 0.01 ） while PR was lower in MMs than that in SMs （P 〈 0. 05）. But no significant difference was found for the expression of Ki-67 or Survivin between two groups. Conclusion The detections of MMP-9, PR, Ki-67 and Survivin are helpful in the clinical diagnosis and early detection of meningioma.

关 键 词：脑膜瘤 免疫组织化学 生物学 

分 类 号：R739.45[医药卫生—肿瘤]