小干扰RNA下调乳腺癌扩增基因3表达对乳腺癌MDA-MB-231细胞转化生长因子-β通路的影响  

Effects of downregulating amplified in breast cancer 3 expression by small interfering RNA on transforming growth factor-beta pathway in breast cancer MDA-MB-231 cells

作　　者：游颜杰[1,2] 毕磊[2] 庄轶轩[1] 邱希辉[3] 张灏[1,2,3] 

机构地区：[1]汕头大学医学院附属肿瘤医院肿瘤研究实验室,广东汕头515041 [2]汕头大学医学院附属肿瘤医院肿瘤研究中心,广东汕头515041 [3]汕头大学医学院附属肿瘤医院中西医结合科

出　　处：《转化医学杂志》2012年第3期144-146,共3页Translational Medicine Journal

基　　金：广东省医学科研基金项目(B2010228);国家自然基金资助项目(81071736;30973508);汕头大学医学院基础与临床科研基金项目(LD030601)

摘　　要：目的观察小干扰RNA(small interfering RNA,siRNA)特异性干扰乳腺癌扩增基因3(amplifiedin breast cancer 3,AIB3)对乳腺癌细胞转化生长因子-β(transforming growth factor-beta,TGF-β)通路关键分子smad2、smad3及转移相关分子基质金属蛋白-9(matrix metalloproteinases-9,MMP-9)表达的影响。方法制备3组靶向AIB3的特异性siRNA分别转染乳腺癌MDA-MB-231细胞,采用逆转录-聚合酶联反应检测siRNA干涉效果以及smad2、smad3与MMP-9的表达水平变化。结果与空白对照组相比,3组合成的靶向特异性siR-NA均可有效抑制AIB3基因表达;干涉AIB3可同时显著下调smad2、smad3及MMP-9的表达水平。结论成功设计有效干扰AIB3的siRNA序列,在乳腺癌细胞中应用siRNA干扰AIB3可有效抑制TGF-β通路和转移相关通路关键分子的表达,干扰AIB3基因表达有可能成为临床治疗乳腺癌转移的有效策略。Objective To investigate the effects of downregulated expression the amplified in breast cancer 3 （ AIB3 ） gene by designed small interfering RNA on expression of smad2, smad3 and matrix metalloproteinases-9 （MMP-9） in breast cancer MDA-MB-231 cells. Methods Three pairs of siRNAs targeting AIB3 were synthesized for transfection in breast cancer MDA-MB-231 cells. Reverse transcriptional polymerase chain reaction （RT-PCR） was performed to evaluate the inhibitory effects on the expression level of AIB3, as well as smad2, smad3 and MMP-9. Results As com- pared with the control, all three pairs of synthetic siRNAs in this study significantly inhibited the ex- pression of AIB3, subsequently as well as smad2, smad3 and MMP-9. Conclusion We successfully designed siRNAs to silence AIB3 expression, which could affect the key molecules in the TGF-β and metastasis-related pathways in breast cancer. Silencing AIB3 by siRNA may provide a promising strategy for clinical therapy of breast cancer.

关 键 词：乳腺癌 小干扰核糖核酸 乳腺癌扩增基因3 转化生长因子-Β 

分 类 号：R737.9[医药卫生—肿瘤]