一种检测发酵乳中嗜酸乳杆菌的定量定性方法  

Study on the Methods of Qualitative and Quantitative Detection L. acidophilus in the Fermented Milk

作　　者：包秋华[1] 李梅花[1] 徐洁[1] 张家超[1] 张和平[1] 

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,内蒙古呼和浩特010018

出　　处：《食品研究与开发》2012年第11期153-155,共3页Food Research and Development

基　　金：内蒙古自治区自然科学基金项目(2011MS1205);973计划前期研究专项(2010CB134502);教育部创新团队发展计划(IRT0967)

摘　　要：根据乳酸菌的16S rDNA基因序列设计嗜酸乳杆菌种特异性引物,应用种特异性PCR和实时荧光定量PCR反应,通过琼脂糖凝胶电泳图谱和实时荧光定量PCR图谱分析,建立快速、准确地检测发酵乳中L.acidophilus的定量和定性方法。According to 16S rDNA gene sequence of lactic acid bacteria,the species-specific primer of L. acidophilus was designed and used to carry out PCR and fluorescent quantitative Real -Time PCR. Through analyzing the electrophoretic pattern and the curve of RT-PCR, a rapid qualitative and quantitative method for the detection of Lactobacillus rhamnos us in the fermented milk was established.

关 键 词：益生菌 嗜酸乳杆菌 种特异性PCR 实时荧光定量PCR反应 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]