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作 者:舒畅[1] 王高华[1] 肖玲[1] 唐记华[1] 王晓萍[1]
出 处:《中华精神科杂志》2012年第6期368-371,共4页Chinese Journal of Psychiatry
摘 要:目的探讨生命早期母爱剥夺对成年雄性大鼠行为和海马神经再生的影响。方法24只雄性大鼠按随机数字表法分为母爱剥夺组(12只)和对照组(12只)。母爱剥夺组大鼠出生后第2~14天每天接受3h母爱剥夺,90~100日龄时采用糖水偏好测验和旷场测验评估大鼠的快感缺乏、焦虑水平和探索能力,BrdU标记并采用免疫组化方法检测大鼠海马齿状回的再生神经细胞。结果(1)母爱剥夺组的糖水偏好[(61.0±5.6)%]、旷场总行程[(3065±895)cm]、内围场地行程[(1994-287)cm]、直立次数[(10.0±4.8)次]均少于对照组[分别为(80.9±3.5)%,(4888±1112)cm,(483±324)cm,(14.9±5.2)次],2组比较差异有统计学意义(t=-10.398,P=0.000;t=-4.425,P=0.000;t=-2.275,P=0.033;t=-2.415,P=0.024)。(2)母爱剥夺组海马齿状回BrdU阳性细胞数[(20.2±7.1)个]少于对照组[(32.8±8.7)个],差异有统计学意义(t=-3.900,P=0.001)。结论生命早期应激会改变成年大鼠的行为和抑制海马齿状回神经再生,可能会导致成年后抑郁易感性增加。Objective To explore the effects of maternal deprivation on behavior and neurogenesis in hippocampal dentate gyrus in adult rats. Methods Twenty-four newborn male rats were randomly divided into two groups, maternal deprivation group and control group. The maternal deprivation group were deprived from their mother 3 hours per day during postnatal day 2 to 14. The anhedonia, spontaneous anxiety level and the exploratory ability in novel environments was assessed with sucrose preference test and open-field test in adulthood( at the age of 90-100 days). Then, rats were sacrificed and hippocampus was isolated. Newly generated ceils in hippocampal dentate gyms were labeled by BrdU and detected by using immunohistochemistry method. Results ( 1 ) The rate of sucrose preference [ ( 61.0 ± 5.6 ) % ], total distance[ (3065 ± 895) cm], central total distance ( 199 ± 287) cml and the frequency of erection [ (10. 0± 4. 8 )times] in maternal deprivation group were significant less than that in the control group [ (80. 9 ±3.5 ) % , ( 4888 ±1112 ) em, ( 483 ± 324 ) em and ( 14. 9 ±5.2 ) times respectively ( t = -10. 398,P=0. 000;t = -4.425,P =0.000;t = -2.275,P =0.033;t = -2.415,P =0.024)1. (2) BrdU-labeled ceils in the dentate gyms in maternal deprivation group (20. 2 ± 7.1 ) were significant less than that in control group ( 32. 8 ±8.7 ;t = - 3. 900, P = 0. 001 ). Conclusions Early life stress could alter behavior and neurogenesis in hippoeampal dentate gyms in adult male rats. It may increase susceptibility to depressive disorders.
分 类 号:R749.4[医药卫生—神经病学与精神病学]
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