重组高产H1N1亚型猪流感疫苗株的构建与鉴定  

GENERATION AND CHARACTERIZATION OF A HIGH-GROWTH REASSORTANT H1N1 INFLUENZA VIRUS FOR DEVELOPMENT OF SWINE VACCINE

作　　者：温峰[1] 于海[1] 黄梦[1] 杨馥如[1] 马继红[1] 周艳君[1] 李泽君[1] 童光志[1] 

机构地区：[1]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2012年第6期1-5,共5页Chinese Journal of Animal Infectious Diseases

基　　金：国家科技支撑计划重点项目(2010BAD04B03);国家国际科技合作项目(2010DFB33920);中央级公益性科研院所基本科研业务费项目(2010JB01);国家自然科学基金(31201916);上海市自然科学基金青年项目(12ZR1453500)

摘　　要：为制备一株猪流感灭活疫苗的候选毒株,本研究利用反向遗传学技术,将A/swine/Guangdong/1/11(H1N1)毒株的HA、NA基因和A/PR/8/34毒株的PB2、PB1、PA、NP、M、NS基因进行重组,成功拯救出鸡胚高度适应性毒株rH1N1。抗原性分析显示rH1N1保留了亲本毒株良好的抗原性。rH1N1接种10日龄鸡胚48 h后,血凝价达到210,表明该毒株能在鸡胚中高水平复制。该毒株为H1N1亚型猪流感灭活疫苗的研制奠定了坚实的物质基础。In the present study,reverse genetics was applied to generate a high-growth reassortant H1N1 subtype influenza virus（rH1N1） for development of an inactivated swine vaccine with good immunogenicity and efficient propagation in eggs.The reassortant H1N1 virus contained HA and NA genes from a novel isolate [A/Swine/Guangdong/1/11（H1N1）] and six internal genes from high-growth A/Puerto Rico/8/34（PR8） virus.The antigenic analysis showed that rH1N1 retained good immunogenicity of its parent viruses.The growth property of rH1N1 was determined in 10-day-old SPF embryonated eggs.All embryonated eggs survived and HA titers of rH1N1 reached to 1024 at 48 h post inoculation.These results indicated that the high-growth reassortant rH1N1 possessed characteristics of the seed virus for development of an inactivated swine influenza vaccine.

关 键 词：猪流感 H1N1亚型 高繁殖力 反向遗传 

分 类 号：S852.659.5[农业科学—基础兽医学]