Mg^(2+)对Bloom综合症解旋酶与G4DNA结合的影响研究  

Study on the effects of Mg^(2+) on the binding between Bloom syndrome helicase and G4DNA

作　　者：骆衡[1,2,3] 蔡明娟[1,2] 陈祥[1,2] 丁玫[1,2] 李坤[1,2] 许厚强[1,2] 

机构地区：[1]贵州大学动物科学学院高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵阳550025 [2]贵州大学动物科学学院贵州省动物遗传育种与繁殖重点实验室,贵阳550025 [3]贵州大学农学院贵州山地农业病虫害重点实验室,贵阳550025

出　　处：《华东师范大学学报（自然科学版）》2012年第6期81-88,共8页Journal of East China Normal University(Natural Science)

基　　金：国家重点基础研究发展计划(2010CB534912);教育部博士点基金(200806570003);贵州省优秀人才省长资金(200822);贵州省国际科技合作计划项目(黔科合外G字[2011]7008号);大学生创新性实验计划项目(贵大国创字(2010)008号)

摘　　要：利用荧光偏振技术检测了Mg2+对G4DNA、BLM-G4DNA复合物和BLM642-1290解旋酶与G4DNA结合的影响.结果表明,G4DNA荧光偏振值随着Mg2+浓度的增加而增加(P<0.01);BLM-G4DNA复合物的荧光偏振值随着Mg2+浓度的增加出现下降—升高—下降的变化趋势(P<0.01);G4DNA与BLM642-1290解旋酶结合的荧光偏振值随着Mg2+浓度的增加而逐渐下降(P<0.01);分析不同Mg2+浓度下两种分子结合的Kd值,发现Mg2+浓度为3.0mmol/L时,BLM642-1290解旋酶与G4DNA最容易结合,表明适量Mg2+浓度会促进BLM642-1290与G4DNA的结合,但会引起两种分子结合的形状、流动性和电荷等性质的改变.这些结果可为进一步研究BLM解旋酶对G4DNA的作用机理提供相关资料.This paper studied the effects of Mg2＋ on G4DNA,BLM-G4DNA complex,and the binding of BLM642-1290 helicase and G4DNA using fluorescence anisotropy technology.The results indicated that the fluorescence anisotropy of G4DNA increased with Mg2＋ concentration（P0.01）;The anisotropy of BLM-G4DNA complex showed varied tendency of decrease-increase-decrease with Mg2＋ concentration（P0.01）;The anisotropy of the binding between the helicase and G4DNA gradually declined following Mg2＋ concentration（P0.01）;The binding was most likely to occur between the helicase and G4DNA when Mg2＋ concentrations was 3.0 mmol/L,which suggested that the suitable amount of Mg2＋ could promote the binding of the helicase and G4DNA but that could lead to the change of the binding properties of two molecules including the shape,flow ability,and charge.These results provided useful data for studying the interaction mechanism between BLM helicase and G4DNA.

关 键 词：BLM解旋酶 G4DNA Mg2+ 荧光偏振技术 

分 类 号：R733.7[医药卫生—肿瘤]