胡黄连中酚类化合物的分离及UPLC-ESI-MS分析  

Isolation of phenolic constituents from Picrorhiza scrophulariiflora Pennell and UPLC-ESI-MS analysis

作　　者：刘时乔[1] 张新鑫[2] 单淇[2] 周福军[2] 侯文彬[1,2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津药物研究院,天津300193

出　　处：《天津中医药》2012年第6期583-587,共5页Tianjin Journal of Traditional Chinese Medicine

基　　金：天津市中药对照品库的建设项目(033187511)

摘　　要：[目的]研究胡黄连中的酚性成分,并对其在胡黄连药材中的含量进行测定。[方法]采用溶剂提取、硅胶柱色谱及葡聚糖凝胶柱色谱等方法分离纯化,根据1H-NMR、13C-NMR和超高效液相色谱与电喷雾质谱(UPLC-ESI-MS)等鉴定化合物的结构,以分离获得的化合物为对照品,采用HPLC-ESI-MS测定其在提取物及药材中的含量。[结果]从胡黄连中获得6个酚性化合物:云杉苷(Ⅰ)、草夹竹桃苷(Ⅱ)、藏黄连酚苷A(Ⅲ)、藏黄连酚苷F(Ⅳ)、香草酸(Ⅴ)和香草乙酮(Ⅵ)。[结论]上述酚性化合物在胡黄连提取物中的含量分别为1.50%、5.62%、3.53%、1.96%、0.78%和3.16%,在药材中含量分别为0.24%、0.90%、0.56%、0.31%、0.13%、0.51%。[Objective] To study the Phenolic constituents in Picrorhiza scrophulariiflora Pennell and determine their contents in the medicinal materials. [Methods] The compounds were isolated by the solvent extract, silica gel and Sephadex LH-20 columns and their structure were elucidated by 1H-NMR, 13C-NMR and UPLC-ESI-MS which were used as analysis standards. Their contents in the extract and medicinal materials were determined by UPLC-ESI-MS. [Results] Six phenolie compounds were obtained and identified as Picein （ I ）, Androsin （]]）, Scrophenoside A（Ⅲ）, Scrophenoside F（Ⅳ）, Vanillie acid （V） and Aeetovanillone（Ⅵ）. [Conclusion] The contents of above compounds in the extract are 1.50%, 5.62%, 3.53%, 1.96%, 0.78% and 3.16%, and the contents in the medicinal materials are 0.24%, 0.90%, 0.56%, 0.31%, 0.13%, 0.51%, respectively.

关 键 词：胡黄连 UPLC—ESI—MS 酚类化合物 分离 

分 类 号：R284.2[医药卫生—中药学]