葡萄糖和镁离子在谷氨酸诱发的神经细胞损伤中的作用  

Effects of glucose and Mg^(2+) in the neurons damaged by glutamate

作　　者：邢虹[1] 何其华[2] 袁兰[2] 徐家鸰[1] 吴本玠[1] 

机构地区：[1]北京医科大学生物物理系,北京100083 [2]北京医科大学医药分析中心,北京100083

出　　处：《中国病理生理杂志》2000年第6期508-511,共4页Chinese Journal of Pathophysiology

基　　金："九五"国家医学科技攻关项目 96 - 90 6 - 0 2 - 2 1

摘　　要：目的和方法 :以激光共聚焦扫描显微镜观察了无葡萄糖和无镁离子细胞外液对中毒剂量谷氨酸引起的大脑皮质神经细胞损伤作用的影响。结果 :无葡萄糖及无镁离子时均可使谷氨酸引起的神经细胞内钙振荡频率及幅度下降 ,但前者可使细胞内基础Ca2 +降低 ,后者可使细胞内基础Ca2 +增加。结论 :无镁可加重谷氨酸引起的细胞内Ca2 +超载 ,在一定条件下无葡萄糖可拮抗谷氨酸及无镁离子的损伤作用。AIM and METHODS: To observe the effects of glucose-free and Mg 2+ -free in the extracellular fluid on the changes of [Ca 2+ ] i in the cerebro-cortical neurons damaged by 1 mmol/L glutamate using laser confocal scanning microscope. RESULTS: Both frequency and amplitude of neuronal calcium oscillation induced by glutamate were lowered in glucose-free and Mg 2+ -free buffers. The basic [Ca 2+ ] i concentration was lowered in the former case , but it was elevated in the latter case. CONCLUSION: Mg 2+ -free aggravates [Ca 2+ ] i overload induced by 1 mmol/L glutamate ,under certain conditions the glucose-free might resist damage role of glutamate and Mg 2+ -free.

关 键 词：谷氨酸盐类 葡萄糖 脑缺血 镁离子 钙离子 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]