河北井陉矿区红花刺槐根围AM真菌与土壤因子相关性  

Relativity Analysis Between AM Fungi and Soil Factors of Robinia hispida from Jingxing Mining Area in Hebei Province,China

作　　者：贺学礼[1] 荣心瑞[1] 牛凯[1] 王雷[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《农业环境科学学报》2012年第12期2381-2388,共8页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(31170488)

摘　　要：为了探明煤矿区土壤AM真菌资源和生态分布特点,2011年8月从河北井陉矿区选取工业园、大兰山和贾庄镇3个样地,以矿区优势植物红花刺槐(Robinia hispida)为目标植物,分0～10、10～20、20～30、30～40cm和40～50cm5个土层采集根围土壤样品,系统研究了AM真菌生态分布及土壤因子的生态作用。共分离鉴定出AM真菌4属21种,其中球囊霉属(Glomus)13种,无梗囊霉属(Acaulospora)6种,多孢囊霉属(Diversispora)和内养囊霉属(Entrophospora)各1种。AM真菌与红花刺槐根系能形成良好共生关系,AM真菌生态分布具有明显空间异质性,最大孢子密度出现在0～20cm土层,最高定殖率多在0～30cm土层。相关性分析发现,孢子密度与易提取球囊霉素、总球囊霉素和土壤碱解氮极显著正相关,与土壤酸性磷酸酶显著正相关;总定殖率与土壤脲酶和有机质极显著正相关。主成分分析表明,土壤脲酶、碱解氮、酸性磷酸酶和碱性磷酸酶是影响井陉矿区AM真菌与植物共生的主要因子。易提取球囊霉素占有机质百分比均值为0.416%,总球囊霉素占有机质百分比均值为0.511%,说明球囊霉素是井陉矿区土壤有机碳库的重要来源。To elucidate AM fungal resources and distribution in coal mine area,soil samples were collected from a depth of 50 cm in the rhi-zosphere of Robinia hispida and were divided into five depth intervals：0～10,10～20,20～30,30～40,40～50 cm in the three sampling sites including Gongyeyuan,Dalanshan and Jiazhuangzhen from Jingxing mining area in Hebei Province.Among 21 AM fungal species were isolat-ed from the tested soil samples,13 species belonged to Glomus,six to Acaulospora,one to Diversispora and one to Entrophospora.Spore den-sity and colonization of AM fungi had significant spatial differences.The highest spore density and colonization were found at 0～20 cm and 0～30 cm soil layer,respectively.Relativity analysis showed that spore density was positively correlated with TG（total glomalin）,EEG（easily extractable glomalin） and soil available N,and was positively correlated with soil acid phosphatase.Colonization intensity had a significantly positive correlation with soil urease and organic matter.Principal component analysis showed that soil urease,available N,acid and alkaline phosphatase were the key factors affecting distributions and activities of AM fungi in the mining area of Jingxing.EEG and TG accounted for 0.511% and 0.416% of soil organic matter.The results supported that glomalin was an important source of soil organic carbon in Jingxing mining area.

关 键 词：AM真菌 土壤因子 红花刺槐 煤矿区 河北井陉县 

分 类 号：X172[环境科学与工程—环境科学]