耐盐菌BF40产表面活性剂特性及其对石油污染盐渍化土壤的修复能力  

Biosurfactant Producing Characteristics of a Salt-tolerant BF40 Strain and Its Remediation in Petroleum Contaminated Saline Soil

作　　者：吴涛[1,2] 依艳丽[1] 谢文军[3] 许杰[4] 姚志刚[2] 李小彬[2] 

机构地区：[1]沈阳农业大学土地与环境学院,沈阳110866 [2]滨州学院生命科学系,山东省黄河三角洲野生植物资源开发利用工程技术研究中心,滨州256600 [3]滨州学院,山东省黄河三角洲生态环境重点实验室,滨州256600 [4]滨州职业学院生物工程学院,滨州256603

出　　处：《农业环境科学学报》2012年第12期2389-2396,共8页Journal of Agro-Environment Science

基　　金：山东省科技攻关计划项目(2009GG10006012);滨州市科技发展计划项目([2010]26);山东省中青年科学家科研奖励基金(2008BS09024)

摘　　要：为强化微生物修复石油污染盐渍化土壤并提供高效产表面活性剂菌种,研究了耐盐菌SerratiaBF40产生物表面活性剂的条件、动力学特征以及对石油污染盐渍化土壤的修复能力。结果表明:BF40产表面活性剂最适碳源为牛肉膏,最适氮源为氯化铵。在30～37℃,pH7.0～9.0范围内产表面活性剂的能力较强。BF40在对数生长期产生表面活性剂,产生方式与细胞生长相关联。在含2.0%NaCl的培养基中,BF40可将发酵液表面张力降低到32.0mN·m-1,EI24达到66.9%。在含盐量为0.22%和0.61%土壤中添加BF40,降解40d后,土壤总石油烃降解率达到50%以上,表明BF40在强化修复石油污染盐渍化土壤中具有很大的应用潜力。To provide high-efficient biosurfactant-producing bacteria and enhance the bioremediation efficiency of petroleum contaminated saline soil,the biosurfactant-producing conditions and dynamics of a salt-tolerant Serratia BF40 strain and its remediation in petroleum con-taminated saline soil were carried out.Results indicated that the optimum carbon and nitrogen sources for biosurfactant production were beef extract and NH 4 Cl,respectively.BF40 strain could produce more biosurfactant at 30~37 ℃ and pH ranged from 7.0 to 9.0.Biosurfactant was produced during the log phase and producing type was correlated with cell growth.Surface activity of fermentation broth was significantly ef-fective in the presence of 2.0% NaCl,and the value of surface tension and EI24 were 32.0 mN·m-1 and 66.9%,respectively.Petroleum degra-dation experiments showed that BF40 strain could remove more than 50% of total petroleum hydrocarbons after 40 days in the soil with 0.22% and 0.60% salinity.These results indicated that BF40 strain had potential used in bioremediation of petroleum-contaminated saline soil.

关 键 词：石油污染 盐渍化土壤 生物表面活性剂 沙雷氏菌 生物修复 

分 类 号：X172[环境科学与工程—环境科学]