饵料对蚯蚓粪细菌群落结构多样性的影响  

Characterization of Bacterial Community Diversity in Various Vermicomposts Produced from Feedstocks

作　　者：陈浩[1,2] 赵海涛[1] 姚振飞 孙红军 胡健[1] 

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225127 [2]江苏省农业科学院食品质量安全与检测研究所,农业部农产品质量安全控制技术与标准重点实验室,江苏南京210014 [3]昆山市土壤肥料站,江苏昆山215300 [4]江苏东宝生物科技有限公司,江苏扬州225231

出　　处：《农业环境科学学报》2012年第12期2500-2505,共6页Journal of Agro-Environment Science

基　　金：农业部十二五公益性农业行业科研专项(201103004);国家自然科学基金(31000939);江苏省农业科技支撑项目(BE2011361)

摘　　要：以牛粪、猪粪和污泥等有机废弃物作为蚯蚓饵料,利用细菌16SrDNAV3区特异扩增及变性梯度凝胶电泳(PCR-DGGE)技术,比较了不同饵料及饵料改变对蚯蚓粪细菌群落结构多样性的影响。结果表明:(1)与所取食的饵料相比,蚯蚓粪细菌群落结构多样性均有不同程度的下降,蚯蚓粪与对应饵料之间细菌群落结构相似性范围为15.3%～37.7%;(2)来自不同饵料的蚯蚓粪细菌群落结构相似性范围为17.2%～39.1%,其中以牛粪和猪粪为饵料时,蚯蚓粪细菌群落结构相似性最高,与对应的饵料细菌群落结构相似性相比,提高了近1倍;(3)当饵料由猪粪改为牛粪后,随着时间延长,蚯蚓粪细菌群落结构多样性逐渐下降并趋于稳定,并且蚯蚓粪与饵料牛粪的细菌群落结构相似性由0d的33.2%逐渐下降到21d的14.8%并趋于稳定。由此可知,饵料的种类及转换可以影响蚯蚓粪细菌群落结构多样性。The earthworms were fed with different feedstocks,such as cattle manure,pig manure and sludge.Then the bacterial community diversity between feedstock and vermicompost were determined by DGGE analysis of PCR-amplified bacterial 16S rDNA V3 region.The re-sults showed that：（1） The diversities of bacterial community in various vermicomposts were all decreased as compared with different feed-stocks.The similarity coefficients of the bacterial community between vermicompost and feedstock were different among all the tested sam-ples,which were from 15.3% to 37.7%（;2） Feeding with different kind of feedstocks resulted in the different similarity coefficient of bacterial community among all the types of vermicomposts,but some vermicompost samples showed similar bacterial community diversity.The highest similarity value was found between two vermicomposts derived from cattle manure and pig manure,respectively,both of which were about one fold higher than their corresponding feedstocks（;3） The bacterial community diversity of fresh vermicomposts declined and tended to be stable with the extension of feeding time after changing the feedstock from pig manure to cattle manure.The bacterial community diversity in feed-stock could be changed after feeding by earthworm.The similarity coefficient between cattle manure and its corresponding vermicomposts was decreased from 33.2% on 0 d to 14.8% on 21 d,and then tended to be stable after 21 d.In sum,our data suggested that the bacterial commu-nity diversity in vermicomposts could be influenced by the types and conversion of feedstocks.This work will help our understanding of the mechanism for disposal of organic wastes using earthworm bioreactor.

关 键 词：饵料 蚯蚓粪 PCR-DGGE 细菌群落多样性 

分 类 号：X172[环境科学与工程—环境科学]