固相萃取结合高效液相制备茶树没食子酸衍生物  

Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant

作　　者：卢忠尉[1] 蒋晓岚[2] 刘亚军[1] 刘莉[2] 高丽萍[1] 夏涛[2] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]安徽农业大学教育部茶叶生物化学与生物技术重点实验室,安徽合肥230036

出　　处：《茶叶科学》2012年第6期494-499,共6页Journal of Tea Science

基　　金：国家自然科学基金(No.30972401;No.31170647和No.31170282);安徽省自然科学基金(11040606M73)

摘　　要：没食子酸衍生物是茶树酚酸中的主要成分,是茶汤中重要的鲜味增强剂,具有抗过敏、抗抑郁和增强认知能力等作用。利用固相萃取(solid-phase extraction,SPE)结合制备高效液相色谱(preparative highperformance liquid chromatography,PHPLC),从茶树鲜叶中分离制备出高纯度的3-O-没食子酰奎尼酸和β-D-没食子酰葡萄糖,并利用LC/TOF-ESI-MS、1H-NMR和13C-NMR对它们的结构进行了鉴定。该方法操作简单、纯化效果好。Gallic acid derivatives are the chief compounds of phenolic acid in tea.They are important umami-enhancing compounds in tea beverage,and possess the effects of inhibiting IgE producton,enhancing cognition-anting depressant.A reversed-phase PHPLC coupled with SPE was developed to purify and to prepare high purity 3-O-galloyquinic acid and β-D-glucogalline from fresh tea leaves.The obtained fractions were successfully identified by LC/TOF-ESI-MS,1H-NMR and 13C-NMR.This method is simple,effective for the preparation of gallic acid derivatives.

关 键 词：没食子酸衍生物 固相萃取 制备高效液相色谱 β-D-没食子酰葡萄糖 3-O-没食子酰奎尼酸 

分 类 号：TS272.7[农业科学—茶叶生产加工] O657.72[轻工技术与工程—农产品加工及贮藏工程]