兼性厌氧菌群对湖滨带底泥有机污染物的降解效应  

Biodegradation of organic contaminants in sediment of littoral zone by facultative anaerobic bacteria

作　　者：张蕊[1,2] 曹蓉[1] 叶春[2] 李春华[2] 

机构地区：[1]河北工程大学城市建设学院,河北邯郸056038 [2]中国环境科学研究院,北京100012

出　　处：《环境污染与防治》2012年第12期61-65,69,共6页Environmental Pollution & Control

基　　金：国家水体污染控制与治理科技重大专项(No.2009ZX07101-009)

摘　　要：研究了兼性厌氧菌群不同接种量(1%(体积分数,下同)、5%、10%、20%、50%)下对太湖湖滨带底泥有机污染物的降解效果及微生物群落结构的影响。结果表明,与对照组相比,接种兼性厌氧菌群能显著提高总有机碳(TOC)、TN的降解率且降解率高,但是TP的降解率波动较大。补充实验证明接种菌液中大量的有机碳、氮源会引起样品中初始浓度升高,建议在使用接种菌液前进行3次无菌水冲洗。综合考虑接种兼性厌氧菌群对湖滨带底泥有机污染物的降解效果,确定其最佳接种量为10%。另外,接种兼性厌氧菌群后其菌群数量和菌活性都有显著提高而严格好氧菌、严格厌氧菌数量均无显著变化;第0天,微生物在接种量为10%时菌活性电子传递体系(ETS)最高。A facultative anaerobic bacteria was inoculated to the sediment sample of littoral zone in Taihu Lake. The inoculation size of facultative anaerobic bacteria was 1%（volume ratio, similarly hereafter）, 5%, 10%, 20% and 50%, respectively. The effect of inoculation size on organic contaminants biodegradation and microbial community structure was investigated. Compared with the control group, inoculating facultative anaerobic bacteria （test groups） could greatly increase the TOC and TN removal rate. While TP removal rate presented relative larger fluctuation. There was large amount of organic carbon and nitrogen containing in inoculum, so the inoculum should be washed three times with sterile water before inoculated in sediment samples. Comprehensively considering the biodegradation of TOC, TN and TP, the optimum inoculation size of facultative anaerobic bacteria was 10%. The facultative anaero- bic bacteria population sizes and bacteria activity ETS value in the test groups were higher than those in the control group. However, aerobic bacteria and anaerobic bacteria population sizes did not change significantly. The highest ETS value was found in group with 10% of inoculation size on day 0.

关 键 词：兼性厌氧菌群 有机污染物 湖滨带 

分 类 号：X172[环境科学与工程—环境科学]