可见光谱结合偏最小二乘法对牛奶游离氨基酸的同时测定  

Visible spectroscopy combined with partial least squares method determination of free amino acids in milk at the same time

作　　者：加列西.马那甫 高珍珍[2] 德娜.吐热汗 杨晓君[4] 

机构地区：[1]新疆农业大学化学工程学院,新疆乌鲁木齐830052 [2]新疆农业大学草业与环境科学学院,新疆乌鲁木齐830052 [3]新疆农业大学数理学院,新疆乌鲁木齐830052 [4]新疆农业大学食品与药学学院,新疆乌鲁木齐830052

出　　处：《计算机与应用化学》2012年第12期1475-1478,共4页Computers and Applied Chemistry

基　　金：新疆维吾尔自治区自然科学基金项目(2011211A028)

摘　　要：甘氨酸、谷氨酸、缬氨酸和苯丙氨酸与硫酸铜发生灵敏的显色反应,形成蓝色配合物,由于最大吸收波长接近,吸收光谱重叠,用传统的光度法难以进行单一组分的测定。本文通过测定甘氨酸、谷氨酸、缬氨酸和苯丙氨酸模拟混合试样在660nm～750 nm波长范围的吸收光谱,采用偏最小二乘法建立校正模型,对其含量进行预测,结合光度法建立同时测定以上氨基酸含量的新方法,方法应用于牛奶样品中甘氨酸、谷氨酸、缬氨酸和苯丙氨酸含量的同时测定。对模拟混合试样,回收率98.46%～98.79%:RPEs为1.67%～2.34%。The sensitive color reaction of glycine, glutamic acid, valine and phenylalanine with copper sulfate to form a blue complex, due to the maximum absorption wavelength close to the absorption spectra overlap more serious, traditional spectrophotometry is difficult to Determination of single components. By measuring the absorption spectra of the mixed sample of glycine, glutamic acid, valine and phenylalanine analog in the 660 nm-750 nm wavelength range, the use of partial least squares method to establish the calibration model to predict its content, combined with spectrophotometry to establish a new method for simultaneous determination of amino acid content, the method is applied to the simultaneous determination of glycine, glutamic acid, valine and phenylalanine content in the analog-mixed samples and milk samples. Analog mixed sample, the recovery rate of 98.46 %-98.79 %; RPEs 1.67 %-2.34 %.

关 键 词：偏最小二乘法 光度法 同时测定 牛奶 游离氨基酸 

分 类 号：TQ015.9[化学工程] TP391.9[自动化与计算机技术—计算机应用技术]