人食管癌间充质干细胞对食管癌细胞株Eca-109侵袭性的影响  

Effect of human esophageal carcinoma mesenchymal stem cells on the invasiveness of esophageal carcinoma cell line

作　　者：朱孝中[1] 刘德森[1] 俞力超[1] 胡嘉波[2] 王晓慧[2] 

机构地区：[1]江苏大学附属医院胸外科,江苏镇江212001 [2]江苏大学基础医学与医学技术学院,江苏镇江212013

出　　处：《实用临床医药杂志》2012年第22期18-20,共3页Journal of Clinical Medicine in Practice

基　　金：江苏大学临床医学科技发展基金(JLY2010165)

摘　　要：目的探讨人食管癌间充质干细胞(hEC-MSCs)对食管癌细胞株Eca-109侵袭性的影响。方法在体外将间质干细胞与食管癌细胞株ECA-109非接触共培养,使用RT-PCR和Western blotting的方法检测间质干细胞对ECA-109细胞株中基质金属蛋白酶-9(MMP-9)和抑癌基因E-cadherin表达的影响。结果间质干细胞可明显上调ECA-109细胞株中的MMP-9表达,显著下调ECA-109细胞株中E-cadherin的表达。结论食管癌间充质干细胞可能参与调节食管癌细胞的侵袭与转移。Objective To detect the effect of human esophageal carcinoma mesenchymal stem cells（hEC-MSCs） on the invasiveness of esophageal carcinoma cell line. Methods Human e- sophageal carcinoma mesenehymal stem cells and esophageal cancer cell line ECA-109 non-contaetly in vitro were co-cuhured to detect the effect of human esophageal carcinoma mesenchymal stem cells on the expression of MMP and E-cadherin of esophageal carcinoma cell lines with the technology of RT- PCR and Western blotting. Results Esophageal mesenchymal stem cells can significantly up-regulate the matrix metalloproteinase-9 （ MMP-9 ） expression of esophageal carcinoma cell line ECA-109, and can significantly lower the E-eadherin expression of esophageal carcinoma cell line ECA-109. Conclu- sion Esophageal mesenchymal stem cells may be involved in the regulation the invasion and metasta- sis of esophageal carcinoma cell.

关 键 词：食管癌间充质干细胞 食管癌细胞株 侵袭性 

分 类 号：R735.1[医药卫生—肿瘤]