主动脉夹层大鼠模型及其力学机制的研究  

Biomechanical properties study of aorta in β-aminopropionitrile-induced rat model

作　　者：张雷[1] 王亮[1] 陆华[1] 林晨[1] 包俊敏[1] 陆清声[1] 景在平[1] 

机构地区：[1]第二军医大学附属长海医院血管外科,上海200433

出　　处：《中华外科杂志》2012年第12期1108-1112,共5页Chinese Journal of Surgery

摘　　要：目的建立β-氨基丙腈（BAPN）诱导的主动脉大鼠模型并对主动脉夹层发生的应力和应变进行研究。方法将29只3周龄Sprague-Dawley幼年大鼠随机分为对照组（12只）和模型组（17只）。模型组大鼠用含0．25％BAPN的饲料喂养6周以建立主动脉夹层模型，死亡大鼠立即解剖，6周后处死存活大鼠及对照大鼠，获取的主动脉标本进行形态学和病理学检测。应力、应变及弹性模量等力学参数通过材料测试仪分析得出。结果模型组9只大鼠死于主动脉夹层破裂。夹层动脉瘤直径为（6．33±1．17）mm，长度为（9．3±5．0）mm。合并主动脉夹层形成的模型组大鼠（模型2组）主动脉直径较对照组和无夹层形成的模型组大鼠（模型1组）明显升高[（6．49±1．20）mm比（1．45±0．11）、（1．25±0．26），F=165．257；P=0．001，P=0．000]，后两组差异无统计学意义（P=0．108）。模型组大鼠主动脉中膜厚度和初始横截面积较对照组增加（F=27．277和27．153，P均为0．000），但模型1组和模型2组间无差异（P=0．540）。最大拉伸长度、拉伸比、极限应力、极限应变、最大伸展系数和弹性模量在模型2组、模型1组、对照组问逐渐降低（F=61．854～227．938，P〈0．01）。结论BAPN可以成功诱导主动脉夹层大鼠模型，为主动脉夹层形成的力学机制研究提供帮助。Objective To investigate thoracic aortic longitudinal elastic strength in β-aminopropionitrile （BAPN） treated rat model of aortic dissection （AD）. Methods Twenty-nine young rats （Sprague-Dawley） were divided into tow groups, control group （ n = 12 ） and BAPN group （ n = 17 ）. Seventeen rats were treated with 0. 25% BAPN mixed in feed for 6 weeks. All the rats were sacrificed in the end of experiment and aorta was harvested for biomechanical and pathological study. Longitudinal elastic strength and stress were detected and analyzed by material testing machine. Elasticity modulus as well as maximum stretching length, draw ratio, maximum load, maximum strength, and maximum extensibility was calculated according to the analysis with thickness and area of aortic media. Results Nine BAPN-treated rats died of aortic dissecting aneurysm rupture during the experiment. The diameter of the aneurysms was （6. 33±1.17） mm and the length was （9 ± 5 ） mm. The maximum diameter significantly increased in BAPN-induced rats with AD （group B2 ） compared with without AD （group B1 ） and control group （（6.49±1.20） mmvs. （1.45 ± 0.11）, （1.25 ± 0.26）; F = 165. 257, P = 0.001 and 0.000, respectively）, but there was no significance between group B1 and control group （P = 0. 108）. Thickness and area of aortic media in BAPN-induced rats significantly increased compared with control group （F=27. 277 and 27. 153, P = 0. 000 and 0. 000, respectively）, but there was no significance of area between group B1 and B2 （P = 0. 540）. Maximum stretching length, draw ratio, maximum load, maximum strength maximum extensibility and elasticity modulus were significantly decreased from group B2, group B1 to control group （P 〈 0. 01, respectively ）. Conclusions This study built a successful model of AD. Biomechanical analysis and the decrease of maximum stretching length, draw ratio, maximum load, maximum strength maximum extensibility and elasticity modulus may explain the formation o

关 键 词：动脉瘤 夹层 生物力学 模型 动物 大鼠 Sprague-Dawley 

分 类 号：R-332[医药卫生]