甲状腺乳头状癌中P14^(ARF)基因启动子甲基化及蛋白的表达及意义  

P14^(ARF) Gene Promoter Methylation and Protein in Papillary Thyroid Carcinoma Tissue and Its Biologic Significance

作　　者：权明明[1] 王依满[2] 陈茜[2,3] 邢萍[1] 赖永峰[1] 李伯友[2] 杨晶金[2] 金晓燕[2] 梁勇[2] 

机构地区：[1]温州医学院第一临床学院肿瘤外科,325000 [2]台州学院医学院附属台州市立医院肿瘤科,318000 [3]台州学院医学院附属台州市立医院病理科,318000

出　　处：《医学研究杂志》2012年第12期43-47,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81072209);台州市科技局基金资助项目(102ky11)

摘　　要：目的探讨甲状腺乳头状癌中P14ARF基因启动子甲基化及蛋白的表达及意义。方法应用QRT-PCR、甲基化特异性PCR(MSP)、免疫组织化学SP法分别检测人甲状腺乳头状癌及正常甲状腺组织标本中P14ARF基因及其蛋白的表达情况和P14ARF的甲基化状况并对比研究其生物学意义。结果 QRT-PCR检测显示甲状腺乳头状癌组织中P14ARFmRNA的表达明显低于对照组;免疫组织化学结果显示P14ARF蛋白在甲状腺乳头状癌中低表达;MSP检测结果示P14ARF启动子在甲状腺乳头状癌中过度甲基化。P14ARF及其蛋白的表达在甲状腺乳头状癌和对照组之间有统计学显著差异(P<0.05)。结论在甲状腺乳头状癌组织中P14ARF基因及其蛋白均呈现低表达,提示P14ARF基因在甲状腺乳头状癌发生发展中起抑癌作用,其低表达和P14ARF基因的过度甲基化有关。Objective To study the significance of papillary thyroid carcinoma P14ARF gene promoter methylation and its protein ex- pression. Methods The P14ARF mRNA and protein expression in human thyroid papillary carcinoma tissues was detected with QRT - PCR,immunohistochemistry SP. The methylation status of P14ARF mRNA were detected with the methylation - specific PCR （MSP）. Results QRT - PCR showed that P14ARF mRNA was significantly lower in papillary thyroid carcinoma than the control group. Immunohis- tochemistry showed that P14ARF protein was lower expression in thyroid papillary carcinoma. MSP showed the existence of P14ARF excessive methylation papillary thyroid carcinoma. P14ARF gene and its protein expression between papillary thyroid cancer and control group had sta- tistically significant difference （P 〈 0.05）. Conclusion In papillary thyroid carcinoma, P14ARF in the mRNA and protein expression level was low,which indicates that it has tumor suppressor role,and this low expression is due to P14ARF gene hypermethylation.

关 键 词：甲状腺乳头状癌 P14ARF 甲基化 

分 类 号：R735.9[医药卫生—肿瘤]