大鼠煤工尘肺及其对认知功能的影响  

Coal Worker′s Pneumoeoniosis and Its Impact on Cognitive Functions in Rats

作　　者：吕敏丽[1,2] 牛小媛[1] 

机构地区：[1]山西医科大学第一附属医院神经内科,030001 [2]山西省大同市第五人民医院,037000

出　　处：《医学研究杂志》2012年第12期149-152,共4页Journal of Medical Research

摘　　要：目的建立煤工尘肺大鼠模型,并探讨其对认知功能的影响。方法取体重为180～200g健康雄性Wistar大鼠100只,随机分为对照组和煤尘肺组。煤尘肺组经气管灌入煤尘生理盐水悬液1ml,对照组灌入生理盐水1ml。分别于8周和20周进行Morris水迷宫实验检测其学习和记忆能力,之后取腹主动脉血做血气分析,取肺、脑组织行HE染色观察形态学变化,并进行肺组织胶原纤维(van gieson,VG)染色观察纤维化程度。结果血气分析发现,煤尘肺组大鼠随着染尘时间延长,PaO2逐渐降低,PaCO2逐渐升高;肺组织纤维化指数随病程进展逐渐增高;煤尘肺组大鼠逃避潜伏期较对照组明显延长(F=51.879,P<0.05),20周大鼠的逃避潜伏期较8周组明显延长(F=14.603,P<0.05);煤尘肺组大鼠穿越平台次数和跨越目标象限时间占整个游泳时间的百分率较对照组大鼠明显减少(F=30.301,P<0.05;F=28.363,P<0.05)。结论煤工尘肺大鼠肺功能降低所引起的慢性缺氧,可能是导致神经组织损伤和认知功能障碍的重要机制。Objective To establish a model of coal workers pneumoconiosis （CWP）, and to explore its impact on cognitive function in rats. Methods Male Wistar rats with weight of 180 -200g were randomly divided into control group and CWP groups. Coal dust sa- line suspension （1ml） were poured into intra- trachea in CWP groups, while physiological saline in control group （1ml）. Respectively, animals were detected learning and memory at the 8th and 20th week employing Morris water maze. Then, abdominal aortic blood was taken for gas analysis, and lung and brain for morphological observation with HE staining, lung for fibrosis with VG （van gieson） staining. Results With prolonged instillation or with CWP development, gradually reduced PaO2 and increased PaCO2 in blood gas analysis, and gradually increased pulmonary fibrosis index were found in pneumoconiosis rats. Escape latency was significantly longer in CWP groups than in control group （ F = 51. 879, P 〈 0.05 ）, and in 20th week than in 8th week （ F = 14. 603, P 〈 0.05 ）. Significant reductions were observed in number of traversing platform and in percentage of stepping across the target quadrant time and total swimming time in CWP rats compared with control ones （ F = 30. 301 ,P 〈 0.05 ; F = 28. 363 ,P 〈 0.05 ）. Conclusion Chronic hypoxia induced by reduced pul- monary function may be a leading pathogenesis of leading to nerve tissue damage and cognitive dysfunction.

关 键 词：煤工尘肺 慢性缺氧 认知功能 

分 类 号：R-332[医药卫生]