六亚甲基二乙酰胺对MEC-1细胞体外粘附、移动和侵袭的影响  

Effects of hexamethylene bisacetamide on in vitro adhesion, motility and invasion of human mucoepidermoid carcinoma cell line MEC-1

作　　者：刘斌[1] 吴军正[1] 司徒镇强[1] 李焰[1] 

机构地区：[1]第四军医大学口腔医学院生物学教研室,西安710032

出　　处：《中华口腔医学杂志》2000年第3期197-199,共3页Chinese Journal of Stomatology

基　　金：国家自然科学基金!( 3 93 70 740 )

摘　　要：目的　研究分化诱导剂六亚甲基二乙酰胺 (hexamethylenebisacetamide,HMBA)对人粘液表皮样癌细胞系MEC 1体外粘附、移动和侵袭的影响。方法　用 2mmol/LHMBA体外诱导培养MEC 1细胞 ,用MTT(thiazalylblue ,噻唑蓝 )比色法测定细胞在重构基底膜Matrigel上的粘附 ,用Millicell PCF培养系统测定细胞的移动性和侵袭性。结果　HMBA诱导的MEC 1细胞在 30min和 90min粘附实验中的粘附率 ,分别下降了 2 6 81%和 31 13% (P <0 0 5 )。HMBA对MEC 1细胞体外移动和侵袭的抑制率分别为 6 3 49%和 6 7 6 5 % (P <0 0 5 )。结论　HMBA对人粘液表皮样癌细胞MECObjective To investigate the effects of hexamethylene bisacetamide (HMBA) on the in vitro adhesion, motility and invasion of human mucoepidermoid carcinoma cell line MEC 1 Methods MEC 1 cells were induced with 2 mmol/L of HMBA in vitro, the adhesion of MEC 1 cells to the reconstituted basement membrane Matrigel was investigated with MTT colorimetric assay; the motility and invasion of MEC 1 cells were assayed using biopore membrane Millicell PCF inserts culture system Results The adhesion of MEC 1 cells treated with HMBA for 30 min or 90 min reduced by 26 47% and 31 13%, respectively; the motility and invasion of MEC 1 cells treated with HMBA reduced by 63 49% and 67 65%, respectively Conclusions HMBA has anti invasion effects on human mucoepidermoid carcinoma cells in vitro.

关 键 词：粘液表皮样癌 六亚甲基二乙酰胺 MEC-1细胞 

分 类 号：R73-3[医药卫生—肿瘤]