不同人结直肠肿瘤细胞系对常用抗肿瘤药物体外化疗敏感性的比较  

Sensitivity of different human colorectal tumor cell lines to commonly used chemotherapeutic drugs in vitro

作　　者：张晓莉[1,2] 师宪平[3] 曹锦萍[1,2] 宋敏敏[1,2] 卢碧燕[1,2] 李文[1,2] 黄兰兰[1,2] 温创宇[1,2] 皮荣标[4] 黄美近[2] 刘焕亮[1,2] 

机构地区：[1]中山大学胃肠病学研究所 [2]中山大学附属第六医院,广东广州570655 [3]广州医学院生物化学教研室,广东广州510182 [4]中山大学药学院,广东广州510080

出　　处：《中国病理生理杂志》2012年第12期2172-2177,共6页Chinese Journal of Pathophysiology

基　　金：广州市科技计划项目(No.2011J5200009);中山大学百人计划科研团队建设项目(No.88000-3281302);广东省科技计划项目(No.2011B080701064);广东省自然科学基金资助项目(No.9151008901000196)

摘　　要：目的:观察5种常用抗肿瘤药物对这些人结直肠肿瘤细胞系的生长抑制作用,探讨5种常用抗肿瘤药物对11株人结直肠肿瘤细胞系的作用强度以及比较其体外敏感性,研究不同抗肿瘤药物对人结直肠癌细胞系HCT116和SW480热休克蛋白27(HSP27)和HSP70表达水平的影响。方法:采用CCK-8(Cell Counting Kit-8)法检测5种常用抗肿瘤药物分别对11株人结直肠肿瘤细胞系的生长抑制效应,计算50%抑制浓度(50%inhibi-tory concentration,IC50)及敏感指数,并比较不同人结直肠肿瘤细胞系对5种抗肿瘤药物的敏感性,Western blotting检测HSP27和HSP70蛋白表达水平。结果:11株人结直肠肿瘤细胞系对5-氟尿嘧啶(5-FU)和奥沙利铂(OHP)均比较敏感,没有明显耐药性;5株人结直肠肿瘤细胞系对丝裂霉素(MMC)敏感,6株中度敏感;除SW1116外的10株人结直肠肿瘤细胞系都对多西紫杉醇(DXL)敏感,而SW1116细胞对DXL表现出明显耐药性;除LS174T和SW1116外的9株人结直肠肿瘤细胞系都对伊立替康(IFL)表现出中度敏感,LS174T细胞对IFL表现敏感,而SW1116细胞对IFL表现出明显耐药性。抗肿瘤药物作用于人结直肠癌细胞系HCT116和SW480使HSP27的表达上调,但HSP70的表达水平变化不明显。结论:LS174T是多药敏感细胞株,SW1116是多药耐药细胞株,5-FU和OHP为广谱抗结直肠肿瘤药物;化疗药物的敏感性及HSP27表达量检测对临床选择化疗药物具有一定的提示意义。AIM： To observe the antitumor effect of 5 commonly used chemotherapeutic drugs on 11 human colorectal cancer cell lines in vitro. METHODS： CCK - 8 method was used to determine the growth inhibitory effects of 5 antitumor drugs, which were expressed as the half growth inhibitory concentration （ IC50 ） and sensitivity index IC50/PPC （ peak plasma concentration） on 11 human colorectal cancer cell lines. The expression variations of heat - shock protein 27 （HSP27） and HSP70 at protein levels in human colorectal tumor cell lines treated with different chemotherapeutic drugs were observed by Western blotting. RESULTS： All the 11 colorectal cancer cell lines were sensitive to 5 - fluorouracil （5-FU） and oxaliplatin （OHP） without drug resistant. Five colorectal cancer cell lines were sensitive to mitomyein （ MMC）, while the other 6 cell lines were moderately sensitive. Ten colorectal cancer cell lines except SW1116 were sensi-tive to docetaxel （DXL）, while SW1116 cells were resistant to DXL. Nine colorectal cancer cell lines except LS174T and SW1116 were moderately sensitive to irinotecan （IFL）, and SW1116 ceils were also resistant to IFL, while LS174T cells were sensitive to IFL. After treated with the antitumor drugs, HSP27 was up - regulated in HCT116 cells and SW480 cells, while the expression of HSP70 didn＇t change. CONCLUSION： IS174T cells are muhidrug- sensitive, while SW1116 ceils are muhidrug - resistant. 5 - FU and OHP are the wide - spectrum anti - colorectal cancer drugs. Determining the sensitivity to chemotherapeutic drugs and the expression level of HSP27 can improve the accuracy in drug selection.

关 键 词：结直肠肿瘤 化学疗法 药物筛选 热休克蛋白70 热休克蛋白27 

分 类 号：R735.3[医药卫生—肿瘤]