ROCK对系统性红斑狼疮患者外周血T细胞黏附和迁移的调控  

Abnormal activity of ROCK contributes to increased adhesion and migration of peripheral blood T cells from patients with systemic lupus erythematosus

作　　者：梁柳琴[1] 陈伟玲[1] 邱茜[1] 詹钟平[1] 叶玉津[1] 杨岫岩[1] 许韩师[1] 

机构地区：[1]中山大学附属第一医院风湿内科,广东广州510080

出　　处：《中国病理生理杂志》2012年第12期2270-2273,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30671951);教育部出国留学回国人员启动基金资助项目(No.教外司2006-331);广东省科技计划基金资助项目(No.2008B080703015;No.2011B080701011)

摘　　要：目的:研究系统性红斑狼疮(SLE)患者外周血T细胞中Rho激酶(ROCK)活化情况,并探讨其对T细胞黏附和迁移的影响。方法:收集SLE患者33例,正常健康人12例和类风湿关节炎患者9例作为对照组。外周血T细胞分离采用RosettSep T细胞提取试剂盒提取,ROCK活性用磷酸化肌球蛋白磷酸酶靶亚基1(MYPT1)蛋白表达来表示,磷酸化MYPT1蛋白检测采用免疫印迹法,T细胞迁移采用Transwell小室测定。结果:新鲜分离的SLE患者外周血T细胞ROCK活性均显著高于正常对照组和类风湿关节炎组(均P<0.05)。SLE患者T细胞体外黏附和迁移能力显著高于正常对照组;ROCK特异性抑制剂Y27632显著抑制SLE患者T细胞黏附和迁移。结论:SLE患者存在T细胞ROCK活化异常;ROCK可能参与调控SLE T细胞的黏附和迁移;抑制T细胞异常的ROCK活性可能有助于SLE的治疗。AIM： To investigate the role of Rho kinase （ROCK） in the regulation of adhesion and migration of the T cells from systemic lupus erythematosus （SLE） patients. METHODS： The T cells were isolated by RosettSep T cell purification kit. ROCK activity was assessed by Western blotting. T cell migration was examined by Transwell chambers. RESULTS ： Compared with the T cells from healthy controls and rheumatoid arthritis patients, the activity of ROCK in ex vivo T cells from SLE patients was significantly increased. In vitro, the adhesion and migration of the T cells from SLE pa- tients were also increased. Furthermore, the adhesion and migration of the T cells from SLE patients were inhibited by a specific ROCK inhibitor Y27632. CONCLUSION： The results indicate that ex vivo T cells from SLE patients exhibit in- creased activity of ROCK. Alteration of ROCK activity may contribute to the abnormal adhesion and migration of T cells from SLE patients.

关 键 词：红斑狼疮 系统性 T淋巴细胞 RHO激酶 

分 类 号：R363[医药卫生—病理学]