乳源免疫调节肽诱导人食管癌Eca-109细胞凋亡的研究  

Immunomodulating peptide from β-casein induced apoptosis in human esophageal carcinoma cell line Eca-109

作　　者：顾芳[1] 秦宜德[1] 桂丽[2] 王巍[1] 唐宜桂[1] 

机构地区：[1]安徽医科大学基础医学院生物化学与分子生物学教研室,合肥230032 [2]安徽医科大学基础医学院综合实验室,合肥230032

出　　处：《安徽医科大学学报》2013年第1期1-4,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30872992);2011年安徽医科大学校基金(编号:0108027201)

摘　　要：目的研究乳源免疫调节肽(PGPIPN)对人食管癌Eca-109细胞生长和凋亡的影响。方法采用MTT法测定PGPIPN对人食管癌Eca-109细胞的作用,用流式细胞仪(FCM)、琼脂糖凝胶电泳检测PGPIPN诱导Eca-109细胞凋亡,用免疫组化SP法检测PGPIPN诱导Eca-109细胞凋亡过程中survivin、caspase-3蛋白的表达变化情况。结果 PG-PIPN对人食管癌Eca-109细胞有明显的生长抑制作用并有剂量依赖性,其增殖抑制作用与诱导细胞凋亡有关,Eca-109细胞经PGPIPN作用后,其survivin蛋白表达下降,caspase-3蛋白表达增强。结论 PGPIPN能诱导人食道癌Eca-109细胞凋亡,这可能与survivin蛋白表达下降,caspase-3蛋白表达增强有关。Objective To investigate the effect of immunomodulating peptide from β-casein（PGPIPN） on the proliferation and apoptosis of human esophageal carcinoma Eca-109 cells.Methods The effect of PGPIPN on Eca-109 cells were evaluated by means of MTT assay.Cells apoptosis was detected by the flow cytometrie assay（FCM） and agarose gel electrophoresis;immunocytochemistry was used to analyze the expression of survivin and caspase-3 in cells during apoptosis induced by PGPIPN.Results PGPIPN could significantly inhibit the proliferation of Eca-109 cells,which had significant dose-dependent relationships.Its inhibition effect contributed to the induced apoptosis;The study showed that survivin proteins expressed and caspase-3 proteins increased in Eca-109 cells.Conclusion PGPIPN can induce apoptosis of Eca-109 cells in vitro.The mechanism of this apoptotic effect is closely related to the survivin proteins and caspase-3 proteins.

关 键 词：乳源免疫调节肽 ECA-109细胞 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤]