黄芪总苷诱导NB4凋亡过程中死亡受体通路分子变化  

Astragalosides-induced apoptosis in NB4 cells involes in molecular changes of the death receptor pathway

作　　者：谢晶[1] 夏瑞祥[1] 

机构地区：[1]安徽医科大学第一附属医院血液内科,合肥230022

出　　处：《安徽医科大学学报》2013年第1期12-15,共4页Acta Universitatis Medicinalis Anhui

摘　　要：目的探讨黄芪总苷(AST)诱导人急性早幼粒细胞白血病细胞株NB4凋亡过程中死亡受体通路的各分子变化,以说明AST是否依赖死亡受体通路参与NB4细胞凋亡发生。方法不同浓度AST(200、300、400μg/ml)处理NB4细胞48 h后,RT-PCR法检测IKKβ、IκBα、NF-κBp65及Fas的mRNA表达变化,比色法检测caspase-8的相对活性。结果不同浓度的AST能够引起IκBαmRNA表达上调、NF-κBp65 mRNA表达下调,caspase-8活性的升高,而IKKβ及Fas的mRNA表达无明显变化。结论 AST诱导NB4细胞凋亡,可能与死亡受体通路上IκBαmRNA表达上调、NF-κBp65 mRNA表达下调,caspase-8活性升高有关。Objective To investigate whether the mechanism of the astragalosides-induced apoptosis in the acute promyelocytic leukemia cell line NB4 associates with activation of the death receptor pathway.Methods NB4 cells were treated with different concentration（200,300,400 μg/ml） of astragalosides for 48 h.The mRNA expression of gene IKKβ,IκBα,NF-κBp65,Fas and the relative activity of caspase-8 were detected by RT-PCR method and chromatometry assay respectively.Results The expression of gene IκBα was up-regulated while gene NF-κBp65 was down-regulated and the expression of gene IKKβ,Fas were not changed obviously.And the astragalosides-induced apoptosis involved in promotion of the relative activity of caspase-8.Conclusion The molecular mechanism of the astragalosides-induced apoptosis in NB4 cells may be associated with regulating of the expression of IκBα,NF-κBp65 mRNA and promotion of the relative activity of caspase-8 that indicates activation of the death receptor pathway.

关 键 词：黄芪总苷 NB4细胞株 细胞凋亡 死亡受体通路 

分 类 号：R733.71[医药卫生—肿瘤] R285.5[医药卫生—临床医学]