蒲公英糖蛋白体内外抗氧化作用研究  

Antioxidant Effect of Glycoprotein from Taraxacum mongolicum in vitro and in vivo

作　　者：贾琳斐[1] 杨颖[1] 李清宇[1] 彭晶[1] 马婷婷[1] 李淼[1] 段玉峰[1] 

机构地区：[1]陕西师范大学食品工程与营养科学学院,西安710062

出　　处：《西北植物学报》2012年第12期2486-2491,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：陕西省自然科学基础研究计划项目(2006B18)

摘　　要：为了探讨蒲公英糖蛋白(TMGP)体内外抗氧化活性,采用H2O2、Fe2+、DPPH.和总抗氧化能力反应体系,检测蒲公英糖蛋白体外抗氧化活性,并与VC进行比较;同时建立D-半乳糖所致衰老小鼠模型,测定并比较TMGP组与模型组的血清、肝脏和脑内MDA含量和SOD、CAT、GSH-Px活性。结果显示:(1)随着TMGP浓度增加,其Fe2+、H2O2和DPPH.清除率均有所增加,当TMGP浓度为1.72mg/mL时,对H2O2、Fe2+清除率分别达到76.8%和55%,接近VC水平;当TMGP浓度为1.5mg/mL时,对DPPH.的清除率达到77%,也与VC水平相当,说明TMGP具有一定的体外总体抗氧化能力。(2)将TMGP按低、中、高剂量分别给小白鼠灌胃,可显著增强小鼠体内血清、肝脏、脑组织中SOD、CAT、GSH-Px活性,降低MDA含量,从而提高衰老小白鼠体内的抗氧化能力。研究表明,TMGP具有明显的体内外抗氧化作用。Abstract：We investigated the antioxidant effect of Taraxacum mongolicum glycoprotein （TMGP） in vitro and invivo. The reaction system of H2O2,Fe2＋,DPPH · and total antioxidant capacity was used to detect the antioxidant activity of TMGP in vitro and was compared with Vc. The contents of MDA and the activi- ties of SOD,CAT,GSH-Px in plasma, liver and brain were respectively measured in aged mice model in- duced by D-galactose. The result showed that. （1） The scavenging capacity of TMGP for H2O2 and Fe2＋ were enhanced as TMGP concentration increased. The H202,Fe2＋ scavenging rates reached 76. 8% and 55% respectively at 1.72 mg/mL TMGP,close to the level of Vc ;When TMGP concentration at 1.5 mg/ mL,the DPPH · scavenging rate reached 77% and it was near Vc;（2）The animal experiment showed that the content of MDA decreased and the SOD,CAT,GSH-Px activities increased in plasma, liver and brain in all TMGP groups,significantly increased the antioxidant capacity of mice. TMGP has obvious antioxidant effect in vitro and in vivo.

关 键 词：蒲公英 糖蛋白 自由基 抗氧化 

分 类 号：Q946-33[生物学—植物学]