成年和老年大鼠脑出血后海马齿状回神经干细胞增殖分化的比较  

Proliferation and differentiation of neural stem cells in hippocampus dentate gyrus in adult and aged rats after intracerebral hemorrhage

作　　者：文玉军[1] 王登科[2] 孙征[1] 刘海洋[1] 张莲香[1] 王效军[1] 秦毅[1] 

机构地区：[1]宁夏医科大学基础医学院人体解剖与组织胚胎学系,宁夏银川750004 [2]宁夏师范学院医学部

出　　处：《中国老年学杂志》2012年第24期5455-5458,共4页Chinese Journal of Gerontology

基　　金：宁夏回族自治区自然科学基金资助项目(NZ0650);宁夏回族自治区教育厅重大科研项目(NJ20090093);宁夏医科大学面上项目;宁夏医科大学重点孵育项目(XZ201001)

摘　　要：目的比较成年和老年大鼠脑出血后海马齿状回神经干细胞(NSCs)的增殖与分化,探讨脑出血后NSCs的变化规律。方法制作大鼠脑出血模型,5-溴脱氧尿核苷(BrdU)腹腔注射标记增殖细胞,用免疫组化法检测大鼠海马齿状回BrdU、神经元核抗原(NeuN)、胶质纤维酸性蛋白(GFAP)阳性细胞数的变化。结果正常组和假手术组大鼠海马齿状回均可见BrdU阳性细胞,成年大鼠明显多于老年大鼠。脑出血后成年和老年大鼠各时间点的BrdU阳性细胞均较正常组和假手术组明显增加,7 d组达到峰值,成年大鼠各时间点均明显高于老年大鼠。正常大鼠海马齿状回可见少量BrdU/NeuN和BrdU/GFAP双标阳性细胞,脑出血后成年和老年大鼠海马齿状回双标阳性细胞数均较正常组明显增加,且老年大鼠BrdU/NeuN双标细胞明显少于成年大鼠。结论脑出血后大鼠海马齿状回NSCs增殖明显,成年大鼠明显强于老年大鼠,且新生细胞分化为神经元的比例也明显高于老年大鼠。Objective To investigate the difference of the proliferation and differentiation of neural stem cells （NSCs） in the hippocampus dentate gyrus between adult and aged rats after intracerebral hemorrhage （ICH）, to explore the variation of NSCs after ICH. Methods ICH models were established by injecting collagenase Ⅶ into the brain of rats. Proliferating cells were labeled by BrdU abdominal cavity injection. Immunohistochemical single and double staining with antibodies against BrdU, NeuN and GFAP were used to determine proliferation and differentiation of hippocampus dentate gyms. Results In non-ICH rats, BrdU positive cells in hippocampus dentate gyrus of adult rats were much more than those of aged rats. After ICH, BrdU positive cells in hippocampus dentate gyrus of rats were significantly in- creased. 7 d group rats had reached its peak. BrdU positive cells in hippocampus dentate gyrus of adult rats were much more than those of aged rats all the time. In non-ICH rats, a little of BrdU/NeuN and BrdU/GFAP double positive ceils were found in the hippocampus dentate gyrus. After ICH, the quantity of double positive cells were increased obviously. BrdU/GFAP double positive cells of aged rats were much more than those of adult rats, but BrdU/NeuN double positive cells of aged rats were less than those of adult rats. Conclusions After ICH, NSCs in hippocampus dentate gyrus of rats are activated. Proliferation and differentiation of NSCs in hippocampus dentate gyrus of adult rats are stronger than those of aged rats.

关 键 词：脑出血 神经干细胞 海马齿状回 

分 类 号：R743.3[医药卫生—神经病学与精神病学]