bFGF对人根尖牙乳头干细胞成脂分化影响的研究  

Effect of bFGF on adipogenic differentiation potential of human stem cells from the apical papilla

作　　者：吴家媛[1,2] 贾谦[1] 何文喜[1] 李玉成[1] 况蓉[1] 倪龙兴[1] 牛忠英[3] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032 [2]遵义医学院附属口腔医院,贵州遵义563003 [3]北京解放军第306医院,北京100101

出　　处：《牙体牙髓牙周病学杂志》2012年第12期686-689,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的:研究碱性成纤维细胞生长因子(bFGF)在体外对人根尖牙乳头干细胞(stem cellsfrom apical papilla,SCAP)成脂分化的影响。方法:采用酶消化法获得原代SCAP,用含有5 ng/mL bFGF的成脂诱导液对其诱导培养不同时间,显微镜观察细胞形态变化、油红0染色观察SCAP成脂分化能力、RT-PCR检测PPAR-γ2 mRNA表达情况。结果:SCAP在含有5 ng/mL bFGF成脂诱导液中培养3周,细胞中脂滴形成数量多于对照组,而且细胞密度也相对较大,细胞体积小,未见拉长、变大等现象。RT-PCR检测显示,诱导培养1周时,实验组与对照组PPAR-γ2 mRNA表达无显著性差异(P>0.05);培养3周时,实验组PPAR-γ2 mRNA表达量明显高于对照组(P<0.05)。结论:bFGF具有促进SCAP成脂分化的潜能。AIM： To investigate the effect of bFGF on the adipogenic differentiation of hunman stem cells from the apical papilla （SCAP） in vitro. METHODS ： SCAP was primarily cultured with enzyme- digest methods. Then SCAP was induced to differentiate to the adipogenic cells with or without bFGF （5ng/mL） in vitro. After 1 and 3 weeks, the induced cells were assessed with cell morphological observation, Oil Red-O staining and the expression of PPAR-~/2. RESULTS ： The density of induced cell in experimental group was higher than that in control group after 3 weeks adipogenic induced. The induced cells were small and did not stretch to be longer in the experimental group. As compared to the control group, the expression level of PPAR-~/2 mRNA in the experimental group induced for 3 weeks was increased distinctly（ P 〈 0.05 ）. The change of PPAR-2 mRNA expression was not observed after 1 week induced （ P 〉 0.05 ）. CONCLUTION ： bFGF can increase the adipogenic differentiation potential of SCAP.

关 键 词：碱性成纤维细胞生长因子 根尖牙乳头干细胞 成脂分化 

分 类 号：R780.2[医药卫生—口腔医学]