非小细胞肺癌组织miRNA-21表达及预后相关性研究  

Expression of miRNA-21 in non-small cell lung cancer tissues and correlation with prognosis

作　　者：刘风林[1] 王新锋[2] 王兴武[3] 宋宝[3] 

机构地区：[1]山东省胸科医院外科,山东济南250062 [2]山东省胸科医院基础实验室,山东济南250062 [3]山东省肿瘤防治研究院基础实验室,山东济南250117

出　　处：《中华肿瘤防治杂志》2012年第18期1397-1399,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨miRNA-21在Ⅰ期非小细胞肺癌组织中的表达及其与复发转移和预后的关系。方法:回顾性分析2002-2006年间本院48例Ⅰ期肺癌手术患者的临床资料,采用定量RT-PCR方法检测上述病例肺癌组织标本中miR-NA-21的表达水平,根据miRNA-21相对表达量进行实验分组。应用χ2检验分析miRNA-21表达与肺癌患者复发转移的关系,应用Kaplan-Meier方法分析miR-21表达与生存的关系。结果:肺癌组织中miRNA-21的表达显著高于正常肺组织。以肿瘤组织与正常组织miR-21表达量比值为2作为分界值,将肺癌患者分为miRNA-21分为高表达组和低表达组。miRNA-21高表达组中,肺癌复发转移率为52.4%(11/21),miRNA-21低表达组中,复发转移率为11.1%(3/27),两组比较差异有统计学意义(P<0.05)。生存分析显示miRNA-21高表达组较之miRNA-21低表达组患者生存期明显降低。结论:miRNA-21高表达与肺癌复发转移具有密切的关系,miRNA-21表达增高提示肺癌患者预后不良。OBJECTIVE：To investigate the expression of miRNA-21 in stage I non-small cell lung cancer （NSCLC） tissues and the correlation with recurrence, metastasis and prognosis. METHODS： Forty-eight patients with sage I NSCLC were involved. Real-time TaqMan miRNA assay was used to analyzed the expression of miRNA-21. The patients were divided into two groups according to the relative expression level of miR-21. The correlation between miRNA-21 ex- pression and recurrence and metastasis was analysed by chi-square test. The correlation between miRNA-21 and survival was analysed by Kaplan-Meier method. RESULTS： miRNA-21 expression in NSCLC tissues was significantly higher than the normal lung tissues. Two fold of miR-21 relative expression between tumor tissues and normal tissues was used as a cut-off value,and the patients were divided into miR-21 high-expression group and miR-21 low-expression group. The re- currence and metastasis rate was 52.4% （11/21） in miRNA-21 high-expression group,and 11.1% （3/27） in miRNA-21 low expression group（P〈0.05）. Kaplan-Meier analysis showed that high level of miRNA-21 was associated with a short survival time. CONCLUSION： High expression of miRNA-21 is associated with lung cancer recurrence and metastasis, miRNA-21 may be a useful prognostic biomarker for NSCLC patients.

关 键 词：癌 非小细胞肺 MIRNA-21 复发转移 预后 肿瘤分期 Ⅰ期 

分 类 号：R734.2[医药卫生—肿瘤]