阿洛伐他汀、替硝唑对兔动脉粥样硬化合并牙周炎斑块中炎症因子的影响  

The Impact of Plaque Inflammatory Factors in Atorvastatin and Tinidazole on Atherosclerosis with Periodontitis Rabbit

作　　者：张元[1] 梁平[1] 侯秀丽[1] 张源明[1] 

机构地区：[1]新疆医科大学第一附属医院心脏中心高血压科,新疆乌鲁木齐市830054

出　　处：《中国动脉硬化杂志》2012年第12期1069-1073,1078,共6页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(30960135)

摘　　要：目的在已建立的动脉粥样硬化合并牙周炎的新西兰大白兔的动物模型基础上,探讨阿洛伐他汀、替硝唑对控制动脉粥样硬化及消除牙周炎中肿瘤坏死因子、白细胞介素1及白细胞介素6的影响。方法 48只雄性新西兰大白兔随机分为4组,每组12只,即对照组、他汀(阿洛伐他汀)组、替硝唑组、联合(阿洛伐他汀+替硝唑)组,后3组以相应药物干预。各组分别按照实验设计进行相应干预处理。至20周末处死动物,取血管,采用荧光定量PCR技术进行定量测定。结果标准曲线参数良好,肿瘤坏死因子、白细胞介素1、白细胞介素6和β-肌动蛋白标准品Ct值与其相应梯度稀释标准品浓度呈良好线性关系(R2>0.990)。经熔解曲线分析,在3种炎症因子中,与对照组相比,联合组检出率最低,两组存在统计学差异(P<0.05);他汀组、替硝唑组检出率位于联合组及对照组之间(P<0.05)。结论阿洛伐他汀及替硝唑可以降低兔动脉粥样硬化合并牙周炎的肿瘤坏死因子、白细胞介素1及白细胞介素6含量,且两种药物联合应用对兔动脉粥样硬化合并牙周炎斑块中肿瘤坏死因子、白细胞介素1及白细胞介素6含量的降低效果更佳,为协同作用,这可能成为动脉粥样硬化合并牙周炎的治疗方法之一。Aim To investigate the effect of atorvastatin and tinidazole control animation atheroselerosis and the elimination of periodontitis levels of tumor necrosis factor （ TNF）, interleukin-1 （ IL-1 ） and interleukin-6 （ IL-6 ） on the basis of animal models of atherosclerotic arteries with merged periodontitis. Methods A total of 48 male New Zealand white rabbits with atherosclerosis and chronic periodontitis were randomly divided into 4 groups （ n = 12 ） ： the control group, the atorvastatin group, the tinidazole group, the atorvastatin ＋ tinidazole group. Every group accepted the corre- sponding treatment. All the animals were sacrificed until 20 weeks, and the blood vessels were taken for quantitative de- termination by using quantitative polymerase chain reaction （PCR） technique. Results The standard curve parame- ters of tumor necrosis factor, interleukin-1, interleukin-6 and β -actin standard Ct values of their corresponding serial dilu- tions of the standard concentration showed a good linear relationship （R2 〉0. 990） by melting the song in three kinds of in- flammatory factors. The atorvastatin ＋ tinidazole group, the lowest detection rate was far lower than in control group （P 〈 0.05 ）, while the detection rate of the atorvastatin group and the tinidazole group （ P 〈 0. 05 ） in the atorvastatin ＋ tinidazole group and control group. Conclusion Atorvastatin ＋ tinidazole can reduce the level of TNF, IL-1 and IL-6 in the rab-bit model with atherosclerosis and chronic periodontitis, which may be one of the methods for prevention and treatment of atherosclerosis and periodontitis.

关 键 词：冠心病 牙周炎 阿洛伐他汀 替硝唑 实时荧光定量PCR 兔 

分 类 号：R363[医药卫生—病理学]