应用基因表达谱芯片研究高果糖大鼠胰岛素抵抗相关基因  

Study on Insulin Resistance-Associated Gene in Fructose-Fed Rats by cDNA Microarray

作　　者：王波[1,2] 李玉明[3] 赵旭燕[3] 蔡绍皙[2] 

机构地区：[1]贵州师范学院化学与生命科学院,贵州省贵阳市550018 [2]重庆大学生物工程学院生物力学与组织工程教育部重点实验室,重庆市400044 [3]武警医学院附属医院心血管病研究所,天津市300162

出　　处：《中国动脉硬化杂志》2012年第12期1103-1108,共6页Chinese Journal of Arteriosclerosis

基　　金：贵州省生物学重点支持学科建设基金资助(2011231)

摘　　要：目的比较正常大鼠和高果糖大鼠骨骼肌组织基因表达差异,探讨胰岛素抵抗的发病分子机制。方法实验大鼠分为正常对照组和高果糖组;从大鼠骨骼肌组织抽提mRNA,经逆转录分别用Cy3、Cy5荧光标记,获得两组动物来源cDNA探针;cDNA探针与4096条大鼠cDNA基因表达谱芯片杂交,扫描,重复2次实验,通过计算机数据处理。结果筛选出胰岛素抵抗差异表达的基因共140条,基因上调有62条,基因下调有78条。结论从骨骼肌组织中筛选出大量的差异表达基因,这些基因可能参加了胰岛素的发生发展过程。Ahn To study the expression of insulin resistance-associated gene and to elucidate the molecular mechanisms in skeletal muscle tissue between fructose-fed rats and normal rats with cDNA microarray. Methods The rats were divided into two groups, one was normal and the other was fructose-rich chow. The mRNA was isolated and pu- rified from skeletal muscle tissue of two groups. The mRNA from two kinds of tissue was reverse transcribed to cDNA with Cy3-dUTP and CyS-dUTP separately to prepare hybridization probes. The mixed probes were hybridized to cDNA microar- ray. The microarray was scanned , analyzed and repeated for two times. Results Among the total 4096 tested genes, 140 genes were differently expressed,62 up-regulated,78 down-regulated. Conclusions Gene expression pro- file is different from skeletal muscle tissue between fructose-fed rats and normal rats. The differential expression of genes may be related to the pathogenesis of insulin resistance.

关 键 词：基因 基因芯片 表达谱 胰岛素抵抗 高果糖大鼠 

分 类 号：R363[医药卫生—病理学]