腹主动脉瘤患者CD4^+ CD28^- T淋巴细胞及Th1细胞因子的变化及临床意义  

Expression Change of CD4^+ CD28^- T Cell and Th1 in Peripheral Blood of Patients with Abdominal Aortic Aneurysm

作　　者：许文平[1,2] 李志樑[2] 邱健[1] 谢志泉[1] 

机构地区：[1]广州军区广州总医院干部四科,广东省广州市510010 [2]南方医科大学珠江医院心内科,广东省广州市510280

出　　处：《中国动脉硬化杂志》2012年第12期1117-1120,共4页Chinese Journal of Arteriosclerosis

基　　金：广东省医科学基金资助(A2005505)

摘　　要：目的探讨CD4+CD28-T细胞及Th1细胞因子在大小腹主动脉瘤患者血清中的数量变化及临床意义。方法腹主动脉瘤患者60例,其中小腹主动脉瘤30例,大腹主动脉瘤30例,对照组30例,采用流式细胞术测定3组受检者外周血CD4+、CD8+、CD4+CD28-T亚群数量及占淋巴细胞的比例,运用酶联免疫吸附法实验(ELISA)检测上述各组人群血浆中γ干扰素、肿瘤坏死因子α表达水平。结果腹主动脉瘤组CD4+CD28-T细胞表达水平显著升高,其中CD4+CD28-T细胞在小腹主动脉瘤组升高最为显著(P<0.05)。腹主动脉瘤患者血浆中γ干扰素、肿瘤坏死因子α表达水平也呈明显升高,其表达水平的升高与腹主动脉瘤直径呈正相关性。结论腹主动脉瘤患者外周血中CD4+CD28-T淋巴细胞升高,升高的CD4+CD28-T淋巴细胞可能通过刺激和促进γ干扰素和肿瘤坏死因子α等Th1炎症因子的分泌,在早期腹主动脉瘤发病机制中起着重要的激活作用,并导致腹主动脉瘤局部慢性炎症迁延。Aim To study the role of CD4 ＋ CD28 - T cell and Thl at the early phase of abdominal aortic aneurysm （AAA）. Methods The total of 60 cases were recruited in the study,including 30 cases of small AAA and 30 cases of large AAA, 30 healthy controls. Levels of peripheral CD4 ＋ CIY28--T cells were determined by FACS, the serum level of IFN-r, TNF-a were measured with enzyme-linked immunosorbent assays （ELISA）. Result Compared with healthy controls, the serum level of CD4 ＋ CD28 - T cells and IFN-r, TNF-a in AAA patients were higher than that in controls （P 〈 0. 05）,the expres- sion levels of IFN-r, TNF-a were positively correlated with the diameter of abdominal aortic. Conclusion Expression levels of CD4 ＋ CD28 - T cells and IFN- r were significantly increased in AAA patients, suggesting a potential role of CD4 ＋ CD28 - T cells and IFN-r on pathophysiology of AAAs, especially during the early development of AAAs.

关 键 词：T淋巴细胞 炎症 免疫 腹主动脉瘤 

分 类 号：R743.134[医药卫生—神经病学与精神病学]